Description
Hieff NGS™ Unique Dual Barcode Primer Kit for MGI is a dedicated supporting kit for DNA library construction based on the MGI high-throughput sequencing platform. Using a two-end connector solution, it includes the UDB Adapter and UDB Primer which used in the second-generation sequencing library construction. There are four kinds of sets, and each contains 96 dual-end unique Barcode labeled UDB Primers used in the second generation sequencing library. Together with the MGI library kit provided by YEASEN, it can build up to 384 dual-end unique Barcode labeled libraries. All the reagents provided in the kit were subjected to strict quality control and functional validation, maximizing the stability and repeatability of the library construction.
Specifications
|
Cat.No. |
13536ES02/04 |
13537ES02/04 |
13538ES02/04 |
13539ES02/04 |
|
Size |
96×2 T/96×4 rxn |
96×2 T/96×4 rxn |
96×2 T/96×4 rxn |
96×2 T/96×4 rxn |
Components
|
Components No. |
Name |
Component volume |
|
|
96×2 rxn |
96×4 rxn |
||
|
13536 |
UDB Adapter |
960 μL |
2×960 μL |
|
UDB Primer 001-096 |
10 μL each |
20 μL each |
|
|
13537 |
UDB Adapter |
960 μL |
2×960 μL |
|
UDB Primer 097-192 |
10 μL each |
20 μL each |
|
|
13538 |
UDB Adapter |
960 μL |
2×960 μL |
|
UDB Primer 193-288 |
10 μL each |
20 μL each |
|
|
13539 |
UDB Adapter |
960 μL |
2×960 μL |
|
UDB Primer 289-384 |
10 μL each |
20 μL each |
|
Storage
This product should be stored at -25~-15℃ for 18 months.
Notes
1. Please operate with lab coats and disposable gloves，for your safety.
2. The concentration of UDB Adapter in this kit is 10 μM, and the amount of adapter used for the individual library construction is adjusted according to the library construction kit and the starting template input.
3. The UDB Adapter provided by this kit is a universal short adapter, and the complete library should be obtained by PCR amplification. UDB Primer provide Barcode sequence tags to distinguish samples during high-throughput sequencing. The concentration of UDB Primer in this kit is 10 μM.
4. There are four kinds of Sets, each set contains 96 dual-end unique Barcode labeled UDB Primers, and four Sets can build 384 dual-end unique Barcode labeled libraries.
5. Do not heat the adapter, it should slowly dissolve at room temperature, the laboratory temperature is best set at 20-25℃. The adapter should avoid repeated freezing and thawing. It is recommended to be stored separately, and can be briefly stored at 4℃.
6. by using the Hieff NGS®Unique Dual Barcode Primer Kit for MGI®. The sequencing library structure is as follows:
7. This product is for research use only.
8. Double Barcode sequence designed for MGI platform is based on the principle of base balance. Every 8 as a group for Barcode 1-48 and Every 4 as a group for Barcode 49-384. In order to achieve the best sequencing quality, when building libraries with different sample numbers, it is recommended to use continuous barcode to construct libraries with more than 8 Barcodes, and then perform machine sequencing after mixing.
Sequence information
UDB Adapter for MGI：
5´-/5Phos/ AGTCGGAGGCCAAGCGGTCTTAGGAAGACAATCAG -3´
5´-TTGTCTTCCTAAGCAACTCCTTGGCTCACAGAACGACATGGCTACGATCCGACTT -3´
Barcode 2 Primer for MGI：
5´-/5Phos/CTCTCAGTACGTCAGCAGTT[Barcode 2]CAACTCCTTGGCTCACAGAAC -3´
Barcode 1 Primer for MGI：
5´-GCATGGCGACCTTATCAG[Barcode 1]TTGTCTTCCTAAGACCGCTTGG-3´
[Barcode 2] represents the 10 bp Barcode 2 sequence and [Barcode 1] represents the 10 bp Barcode 1 sequence.
Plate information
Note: Dual Barcode sequence design for the MGI platform is based on the principle of base balance. For the Barcode 1-48, every 8 barcodes are one group; For the Barcode 49-384, every 4 barcodes are one group. In order to achieve the best sequencing quality, when building libraries with different sample numbers, it is recommended to use continuous barcode to construct libraries with more than 8 Barcodes, and then perform machine sequencing after mixing.
