Description
Product description
Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition.
The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing.
This product is produced by GMP process requirements and provided in a liquid form with the following features.
- High purity (≥ 98%)
- Animal-free production
- Low Endotoxin
- High activity at high salt conditions
- Supported by ELISA Kit
Components
|
Components No. |
Name |
20159ES25 (25 KU) |
20159ES60 (100 KU) |
20159ES80 (1 MU) |
20159ES90 (5 MU) |
|
20159 |
Salt Active UltraNuclease GMP-grade (250 U/μL) |
100 μL |
400 μL |
4 mL |
20 mL |
Specification
|
Expression Host |
Recombinant, expressed in Escherichia coli |
|
Molecular Weight |
24.7kDa |
|
Isoelectric point |
9.61 |
|
Purity |
≥ 99% |
|
Storage Buffer |
25 mM Tris-HCl，5 mM MgCl2，500 mM NaCl，50% glycerol |
|
Unit Definition |
The definition of one activity unit (U) is the amount of enzyme that causes a △A260=1.0 in 30 minutes at 37℃ in the excess of substrate at the certain condition. |
Recommended operating conditions
|
Condition |
Optimal |
Effective |
|
Mg2+/Mn2+ |
10~50mM |
1~100mM |
|
pH |
8 |
6.5 ~10 |
|
Temperature |
37℃ |
4~42℃ |
|
Na+/K+ |
500mM |
300~1200mM |
Recommended Usage
|
Condition |
Suggested usage |
|
Protein |
100U/mL |
|
Cell lysate |
500U/mL |
|
Cell extract |
1000U/mL |
|
Viscosity reduction |
50U/mL |
AAV Production Case
|
Supplier |
Condition (100U/mL, 37℃, 1h) |
HCD residuals（ng/ml) |
|
M* |
400mM NaCl |
1.42 |
|
M* |
400mM NaCl,1% Tween |
0.68 |
|
Yeasen 20159ES |
400mM NaCl |
0.87 |
|
Yeasen 20159ES |
400mM NaCl,1% Tween |
0.07 |
Shipping and Storage
The product should be stored at -25 ~ -15℃ for two years. If the product is opened and has been stored at 4℃ for more than one week, we recommend filtering the product to prevent microbial contamination.
Documents:
Manuals