The use of zebrafish Danio rerio as a model for intestinal damage is well established and the dextran sodium sulfate (DSS)-induced zebrafish IBD model mimics different features of mammalian IBD phenotypes, including neutrophilic inflammation in the intestine, excessive mucus production, increased intestinal lymphangiogenesis and upregulation of pro-inflammatory cytokines and markers ccl20, il1β, il23, il8, mmp9 and tnf-α[1].
Fig. 1 Zebrafish
1 Model building
1.1 Animal Model
3dpf（days post fertilization) Zebrafish
1.2 Material
DSS(Yeasen#60316ES,MW36000-50000)、AB-PAS（Yeasen#60534ES）
1.3 Protocol
- Zebrafish embryos were obtained from natural spawning and raised until 1 day post fertilization (dpf) in an incubator at 28.5℃ in recirculating water (60 μg/mL instant ocean sea salts) and supplemented with methylene blue up to 1 dpf；
- After 1 dpf, use E3 embryo culture medium without methylene blue to culture to 3 dpf；
- Prepare 10% DSS storage solution with E3 medium；
- Dilute DSS with culture medium to the maximum non lethal dose (DSS concentration reference: 0.5%);
- The zebrafish was treated with 0.5% DSS from 3 dpf to 6 dpf, and the indexes were observed.
2 Results
The successful indicators of modeling: the number of neutrophils increased, acidic mucin increased, and the expression of inflammatory factors increased.
- Number of neutrophils: the number of neutrophils increased and moved to the intestine and epithelium;
- Acid mucus protein: It was verified by paraffin pathological section and AB-PAS staining method that the acid mucus protein in zebrafish intestine increased after DSS immersion;
- Inflammatory factor expression: The expression of inflammatory factor in zebrafish intestine increased after DSS treatment[1,2].
Fig. 2 Experimental results of zebrafish modeling by Yeasen DSS (Cat # 60316ES)
3 Links to this resource
- Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557.
- Oehlers SH, et al. Dis Model Mech. 2012 Jul;5(4):457-67.
4 Successful case for modeling with Yeasen DSS
Yeasen provides high-quality DSS (Cat#60316ES): purity (98%), sulfur content 17-19%, free sulfur <0.2%, supported by a large number of literature data.
Table 1 Construction of different types of enteritis models with DSS
|
Model |
Modeling samples |
Modeling plan |
Modeling results |
Use evaluation |
|
acute colitis |
BALB/c mice, female, 6-8 weeks, 25 g |
3%-5% DSS drink freely for 7 consecutive days |
Day 5 appeared, the length of the colon was shortened, HE staining, and the inflammation was obvious |
molding speed is fast and the time is short. Consistent with the characteristics of acute colitis model |
|
C57BL/6 mice, male, 8 weeks, 20 g |
3%-5% DSS by gavage, continuous administration |
Day 5 occurs, colon shortening, weight loss, blood in the stool, diarrhea |
High mold rate and short duration. Consistent with the characteristics of acute colitis model |
|
|
chronic colitis |
C57BL/6 mouse, male, 8 weeks, 22 g |
1-2% DSS by gavage, continuous administration |
Day40 appears, colon shortening, weight loss, blood in the stool, diarrhea |
High molding rate . Consistent with the characteristics of the chronic colitis model |
|
colon cancer |
C57BL/6 mouse, male, 8 weeks, 21 g |
1%-2% DSS ad libitum for 5 days for 3 weeks |
14 weeks with shortened colon length, weight loss, HE staining, and obvious inflammation |
High molding rate . Consistent with colon cancer model characteristics |
5 Product order
Hot-selling product only needs 1/3 of the price of M* with same efficiency，and we keep a large stock.
|
Product Name |
Article Number |
Specification |
|
Dextran Sulfate Sodium Salt (DSS), Colitis Grade MW:36000~50000 |
60316ES25/60/76/80 |
25 g/100 g/500 g/1 kg |
|
Azoxymethane (AOM) |
60751ES03/08/10 |
1 mg/5 mg/10 mg |
|
MolPure® Stool DNA Kit |
18820ES08/50/70 |
5 T/50 T/200 T |
|
MolPure® Mag48 Soil/Stool DNA Kit FA |
18528ES48 |
48 T |
|
MolPure®Mag Soil/Stool DNA Kit |
18526ES20/50/70 |
20 T/50 T/200 T |
|
Hematoxylin and Eosin Staining Kit |
60524ES60 |
2×100 mL |
|
Urine Fecal Occult Blood Test Kit |
60403ES60 |
100T |
|
Alcian Blue Periodic Acid-Schiff (AB-PAS) Staining Solution |
60534ES50/60 |
6×50 mL/6×100 mL/ |
6 Published articles with our reagents
- Zhong D, Jin K, Wang R, ,et al. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26:e2312275. doi: 10.1002/adma.202312275. IF=29.4
- Zhang Y, Tu S, Ji X, Wu J, Meng J, Gao J, Shao X, Shi S, Wang G, Qiu J, Zhang Z, Hua C, Zhang Z, Chen S, Zhang L, Zhu SJ. Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway. Nat Commun. 2024 Feb 13;15(1):1333. doi: 10.1038/s41467-024-45636-x.IF= 16.6
- Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
- Lingjun Tong, Haining Hao, Zhe Zhang,et al.Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556
- Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
- Jingjing Gan, Yuxiao Liu, Lingyu Sun,et al.Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J].Applied Materials Today.2021 Dec;25:101231 IF=10.041
- Mengmeng Xu, Ying Kong, Nannan Chen,et al.Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis[J].Frontiers in Immunology.2022; 13: 855645.IF=7.561
- JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
- Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
- Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
- Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885.IF=7.561
- Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
- Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
- Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076