|
PCR Master Mix |
Length |
Extension |
GC Range |
Dye |
Hot Start |
3’ End |
Fidelity |
Application |
|
≤10-15 kb |
1-10 sec/kb |
30-70% |
Rose red |
√ |
3’-dA |
1× |
Genotyping, Colony PCR, Fast PCR |
|
|
≤5 kb |
30 sec/kb |
40-70% |
Blue |
× |
3’-dA |
1× |
Regular PCR, Genotyping |
|
|
≤5 kb |
30 sec/kb |
40-70% |
Colorless |
× |
3’-dA |
1× |
Regular PCR |
|
|
≤4 kb |
30 sec/kb |
30-70% |
Blue |
√ |
3’-dA |
1× |
Mouse Genotyping |
|
|
|
|
|
|
|
|
|
|
|
|
Direct PCR |
|
|
|
|
|
|
|
|
|
≤10-15 kb |
1-10 sec/kb |
30-70% |
Rose red |
√ |
3’-dA |
1× |
Colony Direct PCR |
|
|
≤1 kb |
30 s/kb |
30-70% |
Blue |
√ |
3’-dA |
1× |
Mouse Direct PCR |
|
|
≤8 kb |
10 s/kb for ≤8 kb |
30-75% |
Colorless |
√ |
3’-dA |
1× |
Blood Direct PCR |
|
|
≤1 kb |
60 s/kb |
40-65% |
Blue |
√ |
3’-dA |
1× |
Plant Direct PCR |
|
|
|
|
|
|
|
|
|
Mouse Tissue/ Cell Lysis Reagent |
|
|
|
|
|
|
|
|
|
|
|
|
High Fidelity PCR |
|
|
|
|
|
|
|
|
|
≤16 kb |
5 sec/kb |
20-80% |
Blue |
√ |
Blunt |
83×Taq |
Premix |
|
|
≤13 kb |
30 sec/kb |
30-60% |
Colorless |
√ |
Blunt |
83×Taq |
Enzyme |
|
|
≤13 kb |
30 sec/kb |
30-60% |
Blue |
√ |
Blunt |
83×Taq |
Premix |
|
|
|
|
|
|
|
|
|
|
|
|
Cloning Kit |
No.of Fragments Ligation |
Fragment Length |
Volume |
Premix |
dsDNA Assembly |
ssDNA Assembly |
|
Name |
|
1-6 |
50 bp-25 kb |
20μL or 6μL |
Yes |
√ |
× |
|
Hieff Clone Universal II One Step Cloning Kit |
Reverse transcription and Real-time qPCR master mix
|
qPCR |
Detection |
Rox |
Sensitivity |
Specificity |
High GC |
Components |
|
Dye-Based |
No/Low/High Rox |
9 |
9 |
9 |
Blue qPCR Mix |
|
|
Dye-Based |
No Rox |
9 |
9 |
8 |
Blue qPCR Mix + Yellow Template Diluent |
|
|
Dye-Based |
Low Rox |
9 |
9 |
8 |
Blue qPCR Mix + Yellow Template Diluent |
|
|
Dye-Based |
High Rox |
9 |
9 |
8 |
Blue qPCR Mix + Yellow Template Diluent |
|
|
Dye-Based |
No/Low/High Rox |
9 |
9 |
9 |
miRNA qPCR + H2O |
|
|
Probe-Based |
No/Low/High Rox |
10 |
9 |
8 |
Master mix |
|
|
|
50× ROX Reference Dye |
|
|
|
Reference Dye |
|
|
|
|
|
|
|
|
|
|
RT-qPCR |
Product Positioning |
Multiplex qPCR |
Sensitivity |
Specificity |
High GC |
Compatible Platforms |
|
Dye-Based |
|
9 |
9 |
8 |
No/Low/High Rox |
|
|
Probe-Based |
√ |
10 |
9 |
8 |
No/Low/High Rox |
|
|
|
|
|
|
|
|
|
|
Reverse transcription |
RT Temperature |
High GC or Complex Template |
RT Time |
gDNA Remover |
for qPCR |
Sub-Application |
|
55℃ |
High |
15 min |
√ |
√ |
|
|
|
37℃ |
Good |
50 min |
× |
√ |
miRNA |
|
|
11155 |
50℃ |
High |
5 min |
√ |
√ |
|
|
37℃ |
Good |
5 min |
√ |
√ |
|
|
|
55℃ |
High |
5 min |
√ |
√ |
PCR, ≤14 kb |
Nucleic Acid Electrophoresis
|
Category |
Cat. No. |
Product Name |
Application |
|
Agarose |
Agarose |
Routine nucleic acid electrophoresis |
|
|
10221ES |
High Sieving Agarose (PCR Grade) |
Suitable for separating DNA fragments of 20 bp-800 bp, comparable to polyacrylamide gel |
|
|
10226ES |
Agarose Tablets (0.5 g/tablet) |
Convenient for routine agarose applications |
|
|
Nucleic Acid Dye |
YeaRed Nucleic Acid Gel Stain (10,000× in Water) |
Water-soluble, with spectral properties similar to EB, excitable at 300 nm UV light |
|
|
DNA Marker |
GoldBand 1 kb DNA Ladder |
250-12000 bp (13 bands) |
|
|
10515ES |
GoldBand 50 bp DNA Ladder |
50-1000 bp (14 bands) |
|
|
GoldBand 100bp DNA Ladder |
100-1500 bp (12 bands) |
||
|
10516ES |
GoldBand 100 bp plus DNA Ladder |
100-3000 bp (14 bands) |
|
|
10517ES |
GoldBand 200 bp DNA Ladder |
200-5000 bp (12 bands) |
|
|
10518ES |
GoldBand 500 bp DNA Ladder |
500-5000 bp (8 bands) |
|
|
GoldBand DL2000 DNA Marker |
100-2000 bp (6 bands) |
||
|
GoldBand DL5000 DNA Marker |
100-5000 bp (9 bands) |
||
|
10505ES |
GoldBand DL10,000 DNA Marker |
100-10000 bp (10 bands) |
|
|
10511ES |
GoldBand Full-Scale DNA Ladder |
100-12000 bp (20 bands) |
|
|
10512ES |
GoldBand DL15000 DNA Marker |
250-15000 bp (7 bands) |
