Hieff Canace™ Plus High-Fidelity DNA Polymerase is based on the Pyroccus Furiosis DNA Polymerase, genetically engineered. The enzyme has a 5'→3' DNA polymerase activity and a 3'→5' exonuclease activity, and its fidelity is 83 times that of Taq DNA polymerase and 9 times that of ordinary Pfu DNA polymerase. Two monoclonal antibodies at room temperature that inhibit polymerase activity and 3'→5' exonuclease activity were added to the enzyme solution, which can easily perform highly specific Hot Start PCR, greatly improving the detection rate of amplification and the specificity of the product. The addition of an extension factor to the enzyme solution gives the enzyme the ability to amplify long fragments, and the length of the amplification of the fragment of interest can be up to 13 kb. This product is equipped with an optima buffer that makes the enzyme suitable for amplification of complex templates. It generates blunt ends in the amplification products.

Applications

Gene cloning; amplification of complex templates DNA; high-throughput library building.

Unit Definition

The activity of ingesting 10 nmol of deoxyribonucleotide as an acidic insoluble with activated salmon sperm DNA as a template/primer at 74°C, within 30 min is defined as 1 U.

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for one year. Please avoid repeated freeze-thaw.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. This product is for research use ONLY!