2× Hieff™ Ultra-Rapid II HotStart PCR Master Mix _ 10167ES

2×Hieff^TM Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg²⁺, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.

Features

Rapid PCR: 1 sec/kb

High GC tolerant 

Dye Plus

Applications

Long PCR

Colony Direct PCR

Genotyping

Specifications

Storage

The 2×Hieff^TM Ultra-Rapid II HotStart PCR Master Mix products should be stored at -25~-15℃ for 2 years.

Performance Display

Rapid and Efficient Amplification of Colonies

Fig. 1 E. coli colony limit extension time amplification test, the extension efficiency of 3 kb fragments amplified by 10167ES can reach 1 sec/kb, 6 kb fragment amplification efficiency can reach 3 sec/kb, and 6-10 kb fragment amplification efficiency can reach 5 sec/kb. M: 10,000 DNA Marker (10505ES).

Fig. 2 10167ES amplifies 1-10 kb Agrobacterium colonies, with a detection rate of 100%, high yield, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.

Rapid and Efficient Amplification of Long Fragments + High GC Bacterial Liquid

Fig. 3 Long fragments + high GC bacterial liquid amplification, the results show that 10167ES has a high amplification yield, a detection rate of 100%, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.

Rapid and Efficient Amplification of Genomic DNA

Fig. 4 10167ES amplifies 1-10 kb mouse gDNA, with high amplification yield, a detection rate of 100%, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.

Rapid and Efficient Amplification of λDNA

Fig. 5 10167ES amplifies 5-15 kb λDNA, with an extension efficiency of 1 sec/kb for fragments within 5-10 kb, and 3 sec/kb for 15 kb fragments. M: 10,000 DNA Marker (10505ES).

Reagent Stability, No Worries with Repeated Freeze-Thaw Cycles

Fig. 6 10167ES remains stable after 100 freeze-thaw cycles, still capable of amplifying 5 kb λDNA and 5 kb human gDNA. M: 10,000 DNA Marker (10505ES).

Fig. 7 10167ES remains stable after being heated at 37°C for 14 days, still capable of amplifying E. coli, Agrobacterium, and yeast colonies. M: 10,000 DNA Marker (10505ES).

Customer Feedback Section

High-Efficiency Amplification of Low GC Fragments

Chinese Academy of Sciences, Center for Excellence in Molecular Plant Sciences

Experimental Information: Target fragment 1,100 bp

Fig. 8 10167 amplifies 1.1 kb E. coli colonies, gene source: Methanobrevibacter smithii (GC 30%), high detection rate, high band brightness, better performance than competitors, correct sequencing results. Extension time is 15 sec/kb. Number of cycles: 30 cycles.

Customer Review: The results with Yeasen were better, with higher band brightness and fewer side bands!

l Rapid Amplification of Colonies

Chinese Academy of Sciences, Center for Excellence in Molecular Plant Sciences

Experimental Information: Target fragment 1,200-1,300 bp

Fig. 9 10167 amplifies 1.2-1.3 kb E. coli colonies, fast amplification speed, high band brightness, better performance than competitors, correct sequencing results. Extension time for 10167 is 3-20 sec/kb, for competitors, it's 20 sec/kb. Number of cycles: 33 cycles.

Customer Review: The sequencing signal was good, and it could be detected. If the time was 1s/kb, I think it's very fast, and the brightness of the gel is quite bright, the effect is good!

Guangdong Institute of Microbiology

Experimental Information: Target Fragment 3,000 bp

Fig. 10 Amplification of a 3 kb (GC 38%) Bacillus cereus colony with 10167, with an ultra-fast amplification speed of up to 1 sec/kb, resulting in high yield and superior performance compared to competitors. The number of cycles was 35.

Customer Review: The effect is excellent, the PCR bands are very bright, and amplification at 1 second per kb is more than sufficient, without the need to extend the time. This saved over an hour compared to other brands, which can improve experimental efficiency!

Zhejiang Agricultural University

Experimental Information: Target fragment 2,500 bp

Fig. 12 10167 amplifies 2.5 kb Agrobacterium colonies, high amplification efficiency, 100% detection rate. Extension time is 10 sec/kb. Number of cycles: 35 cycles.

Customer Review: It was amplified in one go, it's easy to use!