|
Components |
Volume (μL) |
Volume (μL) |
Final Concentration |
|
2× Hieff UniconTM Universal TaqMan multiplex qPCR master mix |
10 |
12.5 |
1× |
|
Primer mix (10 μmol/L) |
x |
x |
0.1-0.5 μmol/L |
|
Probe mix (10 μmol/L) |
x |
x |
50-250 nmol/L |
|
Rox reference dye |
0.4 |
0.4 |
1× |
|
Template DNA/cDNA |
1-10 |
1-10 |
- |
|
ddH2O |
up to 20 |
up to 25 |
- |
Description
Hieff Unicon™ Universal Blue qPCR Master Mix (Probe Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition
This product is a 2× Mix pre-mixed reagent that enables up to four fluorescent quantitative PCR reactions in a single reaction well. This product contains the genetically modified antibody method to hot-start Taq enzyme, greatly improving the amplification sensitivity and specificity. At the same time, this product has deeply optimized the multi-reaction buffer, which can improve the amplification efficiency of the reaction and promote the effective amplification of low-concentration templates. This product can be used for genotyping and multiplex quantitative analysis.
Features
- Strong tolerance of blood impurities
- High sensitivity
- Super storage stability
Applications
- Pathogen detection
- Copy number analysis
- SNP genotyping
Specifications
| Hot Start | Built-in hot start |
| Detection method | Primer-probe detection |
| PCR method | qPCR |
| Polymerase | Taq DNA polymerase |
| Type of sample | DNA |
| Application equipment | Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR. |
Components
| No. | Name |
11211ES03 100T |
11211ES08 500T |
11211ES20 2,000T |
11211ES61 10,000T |
| 11211 |
Hieff Unicon™Universal Multiplex qPCR Master Mix (Probe Based) |
1 mL | 5×1 mL | 20 mL | 100 mL |
PCR Reaction setup
Rox reference dye is optional
Cycle Setup
| CYCLE STEP |
TEMPERATURE |
TIME | CYCLES |
| Initial Denaturation |
95°C | 5 mins |
1 |
| Denaturation Extension |
95°C 60°C |
15 secs 30 secs |
40-45 |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 year.
Figures
Figure 1 High stability: 37℃ thermal acceleration stability
The red line: 37℃ thermal acceleration 0 day; The yellow line: 37℃ thermal acceleration 7 days; The blue line: 37℃ thermal acceleration 14 days
Figure 2 High stability: freeze-thaw stability
The red line: freeze thaw o time; The yellow line: freeze thaw 50 times; The blue line: NTC
Documents:
Citations & References:
[1] Zhang P, Lu S, Liu Z, et al. Transcriptomic and Metabolomic Profiling Reveals the Effect of LED Light Quality on Fruit Ripening and Anthocyanin Accumulation in Cabernet Sauvignon Grape. Front Nutr. 2021;8:790697. Published 2021 Dec 14. doi:10.3389/fnut.2021.790697(IF:6.576)
[2] Xu B, Zhang C, Jiang A, et al. Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation. J Biol Chem. 2022;298(7):102115. doi:10.1016/j.jbc.2022.102115(IF:5.157)