2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye) -10108ES

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SKU: 10108ES03

Size: 1mL 100T
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Sale price$18.00 Regular price$20.00

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Description

2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff™ HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff™ HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments.

Features

  • Convenient, ready-to-use mix
  • Thermostable—half-life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs

Applications

  • Mouse genotyping
  • Hotstart PCR

Specifications

Fidelity (vs. Taq) 1 ×
Hot Start No
Overhang 3'-A
Polymerase Taq DNA Polymerase
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Product Type PCR Master Mix (2×)

Components

Name 10108ES03 10108ES08 10108ES50 10108ES60
2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye) 1 mL 5×1 mL 50×1 mL 100×1 mL

Shipping and Storage

Shipped on ice packs. Store at -20°C with a shelf life of 2 years.

PCR Reaction(50 μL)

Components

Vol.

Conc.

ddH2O

to 50 μL

-

DNA

/

-

Primer F (10 μM)

2 μL

0.4 μM

Primer R (10 μM)

2 μL

0.4 μM

2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye)

25 μL

1×

 

DNA Type

Input

Genomic DNA

50 ng-100 ng

Plasmids 

100 pg-20 ng

cDNA

1-5 μL

 

PCR Cycling

 

Temp.

Time

Cycles

95°C

5 min

1

95°C

30 sec

35

50-60°C

30 sec

72°C

30 sec/kb

72°C

10 min

1

Citations & References:

[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)

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FAQ

The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.