Description
2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff™ HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff™ HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments.
Features
- Convenient, ready-to-use mix
- Thermostable—half-life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
Applications
- Mouse genotyping
- Hotstart PCR
Specifications
| Fidelity (vs. Taq) | 1 × |
| Hot Start | No |
| Overhang | 3'-A |
| Polymerase | Taq DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix (2×) |
Components
| Name | 10108ES03 | 10108ES08 | 10108ES50 | 10108ES60 |
| 2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye) | 1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Figure
Cited from" Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9, Front. Oncol. , 23 June 2022. Molecular and Cellular Oncology, Volume 12 - 2022"
Shipping and Storage
Shipped on ice packs. Store at -20°C with a shelf life of 2 years.
PCR Reaction（50 μL）
|
Components |
Vol. |
Conc. |
|
ddH2O |
to 50 μL |
- |
|
DNA |
/ |
- |
|
Primer F (10 μM) |
2 μL |
0.4 μM |
|
Primer R (10 μM) |
2 μL |
0.4 μM |
|
2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) |
25 μL |
1× |
|
DNA Type |
Input |
|
Genomic DNA |
50 ng-100 ng |
|
Plasmids |
100 pg-20 ng |
|
cDNA |
1-5 μL |
PCR Cycling
|
Temp. |
Time |
Cycles |
|
95°C |
5 min |
1 |
|
95°C |
30 sec |
35 |
|
50-60°C |
30 sec |
|
|
72°C |
30 sec/kb |
|
|
72°C |
10 min |
1 |
[1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.