Fig1. Discovery and development of antibiotics

Mechanism

The bacteriostatic or bactericidal effect of antibiotics is mainly aimed at killing the mechanism that "bacteria have but humans (or other animals and plants) do not"。

Application

The most common application of antibiotics in molecular biology experiments is to screen and maintain cultured prokaryotic or eukaryotic cells carrying resistance genes.The corresponding resistance gene is introduced into the cell by gene recombination technology, so that it can acquire the drug resistance of the resistance gene, so as to achieve the purpose of screening or maintaining growth.

2) Cell culture

Cell contamination is almost an inevitable problem encountered in the process of cell culture. Bacteria and fungi are extremely common pollutants in cell culture experiments. Antibiotics can be used in cell culture experiments to prevent or treat cell contamination by fungi and bacteria.

3) Plant protectant

Fungi and bacteria can cause a variety of plant diseases. Another broad application of antibiotics is as plant protection agents. At a certain dose concentration, it is used alone or in combination to protect plants from the harm of pathogenic infection.

4) Animal intestinal flora research

Animal gastrointestinal microbes can participate in a variety of host metabolic processes and play a crucial role in maintaining the body's healthy homeostasis. Specific types of antibiotics can interfere or regulate the number, types and activities of animal intestinal flora, so as to study the impact of changes in intestinal microorganisms on certain metabolic processes.

Product usage advice

Antibiotics are physiologically active substances, and various antibiotics usually act on pathogenic bacteria at very low concentrations, but the specific working concentration needs to be changed according to cell type, medium, growth conditions, cell metabolic rate and experimental purpose. Therefore, it is recommended to establish a kill curve, that is, a dose-response curve, to determine the optimal screening concentration for the experimental system used for the first time.

Yeasen product performance

At present, the quality of antibiotic products on the market is uneven, and Yisheng Bio is dedicated to provide you with a complete range of antibiotic products.

(1) High-quality raw materials, all antibiotic product raw materials are sourced from high-quality fixed suppliers;

(2) Standardized production, using factory mass production mode;

(3) Wide range of applications, which can be used in the fields of molecular biology and biochemical experimental research of tissue culture;

(4) The cooperation platform covers the whole;

(5) To ensure product quality stability, the difference between batches is controlled within 1%, as shown in Figure 3:

Fig 2. Stability test and effective concentration confirmation between different batches of Geneticin

【Note】G69 and G99 are different batches; the concentration of Geneticin is 8 mg/L, 12 mg/L, and 16 mg/L in turn, and the lowest effective concentration is determined to be 16 mg/L, which can perfectly inhibit miscellaneous bacteria. growth, the performance of the two batches was consistent.

Customer case

Fig 3. Colony growth of E. coli on hygromycin-resistant plates with different concentrations, Yeasen and brand S* have the same effect

【Note】A-C areYeasen hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates, D-F are brand S* hygromycin 20 μg/ml, 50 μg/ml, 100 μg/ml plates.

Ordering product

2.Commonly used antibiotics

Published articles with our reagents

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[13] Zhou YM, et al. BMP9 Reduces Bone Loss in Ovariectomized Mice by Dual Regulation of Bone Remodeling. J Bone Miner Res. 2020 May;35(5):978-993. IF: 5.854

[14] Liu F, et al. CDK7 inhibition suppresses aberrant hedgehog pathway and overcomes resistance to smoothened antagonists. Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12986-95. IF: 9.412

[15] Liu C, et al. A programmable hierarchical-responsive nanoCRISPR elicits robust activation of endogenous target to treat cancer. Theranostics. 2021 Oct 11;11(20):9833-9846. IF: 8.579

[16] Zhu G, Yu J, Sun Z, et al. Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma[J]. Cell death & disease, 2021, 12(8): 1-9. IF: 8.469

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