Molecular cloning is used in nearly every laboratory. Restriction endonucleases are a crucial component in molecular cloning experiments, but people are also often bothered by various problems with restriction endonucleases experiments. Common problems are as follows:

• There are so many types of restriction endonucleases, it is difficult to choose.
• Miscleavage, random cleavage, or incomplete enzymatic cleavage.
• The rate of digestion is sluggish; it may take one hour or even overnight.
• Additionally, Multiple enzyme digestion experiments also need to choose a variety of enzyme digestion bTo

To prove with ide you a more thorough grasp of restriction endonucleases, the following will briefly explain what restriction endonucleases are and how various endonucleases are classified in answer to the first question. The Yeasen FuniCut™ series of Fast Restriction Endonucleases may readily resolve the second through fourth problems through strain modulation and an improved procedure!

1. What is Restriction Endonuclease?
2. Restrictions endonuclease nomenclature
3. Restrictions on endonuclease classification
4. Yeasen FuniCut™ Fast Restriction Endonucleases

1. What is Restriction Endonuclease?

Restriction endonucleases are a class of enzymes that can recognize specific nucleotide sequences in double-stranded DNA molecules and cut phosphodiester linkage in DNA chains at specific sites.

Different restriction endonucleases will recognize different DNA sequences, They can cut DNA inside the recognition sequence or at a place not far from the recognition sequence, resulting in various products, as illustrated in Figure 1. BamHI forms sticky ends, KpnI forms 3'ticky ends, and EcoRV forms blunt ends.

Figure 1. Schematic diagram of BamHI, KpnI, EcoRV digestion

2. Restrictions endonuclease nomenclature

The genus name, species name, bacterial strain or serotype, and the order of discovery are the primary factors used to name restriction endonucleases. The name guidelines are displayed in Table 1 below using BstEII as an example:

Table 1. Restrictions endonuclease nomenclature

3. Restrictions on endonuclease classification

According to the complexity of the structure, the mode, of action and the difference between the cofactors, restriction enzymes can be divided into four categories. The following table 2 summarizes the characteristics and typical enzymes of each category.

Table 2. The variations among several restriction endonucleases

As indicated in Table 3 below, it may also be split into three groups based on the similarities and differences between the recognition and digestion sites.

Table 3. Comparative analysis of Isoschizomer, Neoschizomer, and Isocaudarner

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4. Yeasen FuniCut™ Fast Restriction Endonucleases