Hifair^TM V2 Multiplex One Step RT-qPCR Probe Kit（UDG Plus） is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.

In this kit, the first strand cDNA was efficiently synthesized by heat-resistant Hifair^TM Reverse Transcriptase and quantitatively amplified by UNICON^TM HotStart Taq DNA Polymerase. The kit mainly contains optimized MP buffer, enzymes mix, GC-Enhancer etc. The buffer solution already contains Mg²⁺ and dNTP. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction. The dUTP/UDG system was added to effectively prevent the risk of aerosol contamination.

• good versatility：It is suitable for qPCR amplification and detection of nucleic acids of pathogenic microorganisms, human genomes, plants and other species；
• High sensitivity：The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL；
• dUP/UDG anti-pollution system：dUTP/ Heat-labile UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results；
• Support fast program: compatible with fast program, 40min can produce results;
• Super stability: stabel at 37℃ for 14 days,repeated freezing and thawing 10 times.

Partial performance display

High sensitivity

1）4 respiratory targets were detected

2）3 heavy pig diarrhea targets