Keterangan
Interleukin 1 alpha (IL-1 alpha ), IL-1 beta, and IL-1 receptor antagonist (IL-1ra) are members of the IL-1 family. IL-1 alpha and IL-1 beta are agonists that bind the functional IL-1 receptor I (IL-1 RI), which then recruits the IL-1 receptor accessory protein (IL-1 R AcP/IL-1 R3) and initiates signal transduction pathways that include the NF-kappa B. JNK/AP-1, and p38 MAP kinase pathways. Both IL-1 alpha and IL-1 beta also bind the non-signaling IL-1 RII that functions as a decoy receptor. IL-1ra is a receptor antagonist that binds both IL-1 RI and II with equal affinity as the IL-1 alpha and beta. Binding of IL-1ra to IL-1 RI does not initiate signal transduction pathways. IL-1 alpha and beta are pro-inflammatory cytokines that play an important role in host defense. The imbalance between IL-1 ( alpha and beta ) and IL-1ra can influence the development of various pathologic conditions associated with chronic inflammation and tissue destruction. Pre-clinical and clinical studies have indicated that IL-1ra may have therapeutic potential for the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.
Product Properties
|
Synonyms |
IL-1RN, IRAP, IL1 Inhibitor |
|
Accession |
P25086 |
|
GeneID |
60582 |
|
Source |
E.coli-derived Rat IL-1RA, His27-Gln178. |
|
Molecular Weight |
Approximately 17.4 kDa. |
|
AA Sequence |
HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 96% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/mL, corresponding to a specific activity of > 6.7 × 103 IU/mg in the presence of 50 pg/mL rRtIL-1α. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1 μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
Pembayaran & Keamanan
Informasi pembayaran Anda diproses dengan aman. Kami tidak menyimpan detail kartu kredit atau memiliki akses ke informasi kartu kredit Anda.
Pertanyaan
Anda mungkin juga menyukai
FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
Aplikasi tertentu mungkin memerlukan hak kekayaan intelektual pihak ketiga tambahan.
