Description
Hieff® Fast T4 DNA Ligase is a dedicated single-enzyme product designed for the ligation of DNA fragments and adapters during Next Generation Sequencing (NGS) library preparation. Validated for high-throughput sequencing, this product boasts superior quality. With its high-efficiency ligation capabilities, it is particularly well-suited for joining complex nucleic acid fragments, such as the Ω adapters used on the BGI platform.
FeaturesCompatible with multiple samples
High ligation efficiency
ApplicationsMolecular cloning vector construction
NGS adapter ligation
Specifications|
Source |
Bacteriophage T4 |
|
Molecular Weight |
94.77 kDa |
|
Concentration |
400 U/µL |
|
Unit Definition |
In a 20-microliter ligation reaction mixture, the definition of one unit of activity (U) is the amount of enzyme necessary to catalyze the joining of over 50% of DNA fragments derived from 6 micrograms of λDNA that has been digested with Hind III, when incubated at 16 degrees Celsius for a period of 30 minutes
|
|
Components No. |
Name |
10299ES40 |
10299ES42 |
|
10299-A |
Hieff® Fast T4 DNA Ligase (400 U/µL) |
100 μL |
1 mL |
|
10299-B |
10×T4 DNA Ligase Buffer |
250 μL |
5 × 500 μL |
This product should be stored at -25 ~ -15℃ for 2 years.
FiguresTable 1 Library Yield of Different Types of Samples
Note: Constructing NGS libraries using three types of samples: gastrointestinal microbial gDNA, cfDNA, and FFPE HD200 gDNA, to test the library construction capability of Yeasen's Fast T4 DNA Ligase compared to brand N*.The results show that Yeasen's Fast T4 DNA Ligase demonstrates higher efficiency and yield in library construction across different sample types.
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