Description
Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Hieff Unicon™ Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.
Features
- Built-in visual indicator: It contains an inert blue dye that does not interfere with real-time PCR and provides higher visibility in tubes or Wells.
- High specificity: It contains Taq DNA polymerase and is tightly controlled by an antibody-mediated heat-priming mechanism, thereby avoiding nonspecific amplification.
- Wide instrument compatibility: It is compatible with most qPCR instruments and eliminates the need to adjust the concentration of ROX on different instruments
Applications
- Gene Expression
Specifications
| Concentration | 2× |
| Detection method | SYBR Green dye |
| PCR method | qPCR |
| Polymerase | Taq DNA polymerase |
| Type of sample | DNA |
| Application equipment | Most qPCR instruments |
| Product Type | Premix for real-time fluorescence quantitative PCR |
| Apply to (application) | Gene Expression |
Components
| Components No. | Name | 11184ES03 | 11184ES08 | 11184ES50 | 11184ES60 |
| 11184 |
Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) |
1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Shipping and Storage
The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.
Figures
Figure 1. High sensitivity: the ability to detect a single copy.
Figure2. High resolution & Excellent reproducibility of duplicate wells.
Figure3. Excellent detection rate and specificity: broadly suited for the amplification of 30-70% GC.
Cited from "SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4"
Cited from "A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens. Cell . 2020 Sep 3;182(5):1109-1124.e25. doi: 10.1016/j.cell.2020.07.020. "
[1] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[2] Wang S, Li Y, Zhong L, et al. Efficient gene editing through an intronic selection marker in cells. Cell Mol Life Sci. 2022;79(2):111. Published 2022 Jan 31. doi:10.1007/s00018-022-04152-1(IF:9.261)
[3] An LL, Zhao X, Gong XY, et al. Promoter Binding and Nuclear Retention Features of Zebrafish IRF Family Members in IFN Response. Front Immunol. 2022;13:861262. Published 2022 Apr 6. doi:10.3389/fimmu.2022.861262(IF:7.561)
[4] Zhao Y, Wang HP, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions. Bioresour Technol. 2021;319:124150. doi:10.1016/j.biortech.2020.124150(IF:7.539)
[5] Shu C, Wang L, Zou C, et al. Function of Foxl2 and Dmrt1 proteins during gonadal differentiation in the olive flounder Paralichthys olivaceus [published online ahead of print, 2022 Jun 16]. Int J Biol Macromol. 2022;215:141-154. doi:10.1016/j.ijbiomac.2022.06.098(IF:6.953)
[6] Wang S, Huang J, Liu F, et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021;14:7107-7130. Published 2021 Dec 20. doi:10.2147/JIR.S344990(IF:6.922)
[7] Wang J, Hu R, Wang Z, et al. Establishment of Immortalized Yak Ruminal Epithelial Cell Lines by Lentivirus-Mediated SV40T and hTERT Gene Transduction. Oxid Med Cell Longev. 2022;2022:8128028. Published 2022 Mar 25. doi:10.1155/2022/8128028(IF:6.543)
[8] Liu W, Guan Y, Qiao S, et al. Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice. Oxid Med Cell Longev. 2021;2021:9942090. Published 2021 Aug 6. doi:10.1155/2021/9942090(IF:6.543)
[9] Qian Z, Liu C, Li H, et al. Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages. Biomedicines. 2022;10(5):1006. Published 2022 Apr 27. doi:10.3390/biomedicines10051006(IF:6.081)
[10] Zhao X, Huang Y, Li X, et al. Full integration of nucleic acid extraction and detection into a centrifugal microfluidic chip employing chitosan-modified microspheres [published online ahead of print, 2022 Jun 27]. Talanta. 2022;250:123711. doi:10.1016/j.talanta.2022.123711(IF:6.057)
[11] Xu X, Wang H, Li X, Duan X, Wang Y. A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer. Aging (Albany NY). 2022;14(11):4858-4873. doi:10.18632/aging.204116(IF:5.955)
[12] Li F, Hu X, Wu J. Daidzein Activates Akt Pathway to Promote the Proliferation of Female Germline Stem Cells through Upregulating Clec11a [published online ahead of print, 2022 Jun 3]. Stem Cell Rev Rep. 2022;10.1007/s12015-022-10394-0. doi:10.1007/s12015-022-10394-0(IF:5.739)
[13] Ru M, Wang W, Zhai Z, et al. Nicotinamide mononucleotide supplementation protects the intestinal function in aging mice and D-galactose induced senescent cells. Food Funct. 2022;13(14):7507-7519. Published 2022 Jul 18. doi:10.1039/d2fo00525e(IF:5.396)
[14] Ye M, Gao R, Chen S, et al. Downregulation of MEG3 and upregulation of EZH2 cooperatively promote neuroblastoma progression. J Cell Mol Med. 2022;26(8):2377-2391. doi:10.1111/jcmm.17258(IF:5.310)
[15] Wang S, Wang X, Shao Y, et al. Synthesis and evaluation of 3-(phenylethynyl)-1,1'-biphenyl-2-carboxylate derivatives as new HIF-1 inhibitors. Bioorg Chem. 2021;116:105298. doi:10.1016/j.bioorg.2021.105298(IF:5.275)
[16] Guan X, Meng X, Zhu K, et al. MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway. Cell Death Discov. 2022;8(1):84. Published 2022 Feb 26. doi:10.1038/s41420-022-00881-1(IF:5.241)
[17] Liu H, Wang LL, Xu QH, et al. UHRF1 shapes both the trophoblast invasion and decidual macrophage differentiation in early pregnancy. FASEB J. 2022;36(4):e22247. doi:10.1096/fj.202101647RR(IF:5.192)
[18] Chen M, Wang J, Lin L, et al. Synergistic Regulation of Metabolism by Ca<sup>2+</sup>/Reactive Oxygen Species in Penicillium brevicompactum Improves Production of Mycophenolic Acid and Investigation of the Ca<sup>2+</sup> Channel [published correction appears in ACS Synth Biol. 2022 Apr 15;11(4):1705]. ACS Synth Biol. 2022;11(1):273-285. doi:10.1021/acssynbio.1c00413(IF:5.110)
[19] Zhao X , Li X , Yang W , Peng J , Huang J , Mi S . An integrated microfluidic detection system for the automated and rapid diagnosis of high-risk human papillomavirus. Analyst. 2021;146(16):5102-5114. doi:10.1039/d1an00623a(IF:4.616)
[20] Chen H, Jiang Y, Liu R, et al. Curcumin Derivative C66 Suppresses Pancreatic Cancer Progression through the Inhibition of JNK-Mediated Inflammation. Molecules. 2022;27(10):3076. Published 2022 May 11. doi:10.3390/molecules27103076(IF:4.412)
[21] Su W, Qiu J, Mei Y, Zhang XE, He Y, Li F. A microfluidic cell chip for virus isolation via rapid screening for permissive cells [published online ahead of print, 2022 May 2]. Virol Sin. 2022;S1995-820X(22)00075-X. doi:10.1016/j.virs.2022.04.011(IF:4.327)
[22] Ding J, Mei S, Cheng W, Ni Z, Yu C. Curcumin treats endometriosis in mice by the HIF signaling pathway. Am J Transl Res. 2022;14(4):2184-2198. Published 2022 Apr 15. (IF:4.060)
[23] Zhang XY, Shen HH, Qin XY, et al. IL-27 promotes decidualization via the STAT3-ESR/PGR regulatory axis. J Reprod Immunol. 2022;151:103623. doi:10.1016/j.jri.2022.103623(IF:4.054)
[24] Zhu Q, Muyayalo KP, Xu QH, Wang J, Wang H, Liao AH. Prunella vulgaris can improve the pregnancy outcomes of experimental autoimmune thyroiditis rats by inhibiting Th1/Th17 immune responses. J Reprod Immunol. 2022;149:103469. doi:10.1016/j.jri.2021.103469(IF:4.054)
[25] Yang Q, Guo K, Zhou X, et al. Histopathology, antioxidant responses, transcriptome and gene expression analysis in triangle sail mussel Hyriopsis cumingii after bacterial infection. Dev Comp Immunol. 2021;124:104175. doi:10.1016/j.dci.2021.104175(IF:3.636)
[26] Liu Y, Wan HH, Tian DM, et al. Development and Characterization of High Efficacy Cell-Penetrating Peptide via Modulation of the Histidine and Arginine Ratio for Gene Therapy. Materials (Basel). 2021;14(16):4674. Published 2021 Aug 19. doi:10.3390/ma14164674(IF:3.623)
[27] Fu Y, Wang X, Zhang L, Ren Y, Hao L. Allograft inflammatory factor-1 enhances inflammation and oxidative stress via the NF-κB pathway in diabetic kidney disease. Biochem Biophys Res Commun. 2022;614:63-69. doi:10.1016/j.bbrc.2022.04.089(IF:3.575)
[28] Zhao Y, Wang XQ. VvMYB1 potentially affects VvTOR gene expression by regulating VvTOR promoter and participates in glucose accumulation. J Plant Physiol. 2022;272:153668. doi:10.1016/j.jplph.2022.153668(IF:3.549)
[29] Cheng Y, Zheng L, Yang C, Zhang W, Wang H. Propofol inhibits proliferation and migration of glioma cells by up-regulating lncRNA GAS5. Toxicol In Vitro. 2022;80:105321. doi:10.1016/j.tiv.2022.105321(IF:3.500)
[30] Yang B, Wang F, Zheng G. Transmembrane protein TMEM119 facilitates the stemness of breast cancer cells by activating Wnt/β-catenin pathway. Bioengineered. 2021;12(1):4856-4867. doi:10.1080/21655979.2021.1960464(IF:3.269)
[31] Yang X, Liu X, Song F, et al. Seasonal expressions of GPR41 and GPR43 in the colon of the wild ground squirrels (<em>Spermophilus dauricus</em>). Eur J Histochem. 2022;66(1):3351. Published 2022 Jan 21. doi:10.4081/ejh.2022.3351(IF:3.188)
[32] Tang Z, Yuan X, Bai Y, et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (<em>Ondatra zibethicus</em>). Eur J Histochem. 2022;66(2):3398. Published 2022 May 2. doi:10.4081/ejh.2022.3398(IF:3.188)
[33] Zhu P, Chen Y, Wu F, Meng M, Ji K. Expression and promoter analysis of MEP pathway enzyme-encoding genes in Pinus massoniana Lamb. PeerJ. 2022;10:e13266. Published 2022 Apr 12. doi:10.7717/peerj.13266(IF:2.984)
[34] Zhai L, Chen W, Cui B, Yu B, Wang Y, Liu H. Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer. Tissue Cell. 2021;73:101611. doi:10.1016/j.tice.2021.101611(IF:2.466)
[35] Jiang J, Cai M. Cardamonin Inhibited IL-1β Induced Injury by Inhibition of NLRP3 Inflammasome via Activating Nrf2/NQO-1 Signaling Pathway in Chondrocyte. J Microbiol Biotechnol. 2021;31(6):794-802. doi:10.4014/jmb.2103.03057(IF:2.351)
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.