Description
UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.
UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.
Features
- Wide range of applications: degrade all forms of DNA and RNA
- High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg
- Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
- Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
- Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
- GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture
Application
- Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
- Reduction of viscosity caused by nucleic acids
- Sample preparation in electrophoresis and chromatography
- Prevention of cell clumping
Specification
| Expression Host | Recombinant E. coli with UltraNuclease gene |
| Molecular Weight | 26.5 kDa |
| Isoelectric point | 6.85 |
| Purity | ≥ 99% (SDS-PAGE) |
| Storage Buffer | 20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin |
| Unit Definition | The definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides). |
Components
| Components No. | Name | 20157ES25 (25 KU) | 20157ES60 (100 KU) | 20157ES80 (1 MU) | 20157ES90 (5 MU) |
| 20157 | UltraNuclease GMP-grade (250 U/μL) | 100 μL | 400 μL | 4 mL | 20 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.
Figures
- Quality
|
No. |
Test Items |
Competitor |
Yeasen |
|
1 |
Source |
E. coli |
E. coli |
|
2 |
Activity |
≥ 250 units/μL |
250-300 units/μL |
|
3 |
Specific Activity |
≥1.1×106 U/mg |
≥1.5×106 U/mg |
|
4 |
Purity |
≥ 99%（SDS） |
≥ 99%（HPLC） |
|
5 |
Endotoxin |
< 0.25EU/1000U |
< 0.25EU/1000U |
|
6 |
Protease |
None Detected |
None Detected |
- Reaction conditions recommend
Table 1. The optimal reaction conditions for UCF.ME™ UltraNuclease
| Factor | Optimum Condition | Effective condition |
| Mg2+ | 1-5mM | 0-10mM |
| pH | 8-9 | 6-10 |
| Temperature | 37℃ | 0-42℃ |
| Na+/K+ | 0-20mM | 0-150mM |
| PO43+ | 0-10mM | 0-100mM |
| EDTA | 0-0.5mM | 0-5mM |
| Triton®X-100 | 0%-0.4% | 0%-1% |
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