Synonyms

NAG-1; MIC-1;GDF-15; PLAB

Source

Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Arg193-Val308.[Accession | G7PWZ3]

Source

The protein has a predicted MW of 14.15 kDa. The protein migrates to 15-18 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE

Activity

ELISA Data: Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA .Immobilized Cynomolgus GDF15, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.

Formulation

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).