Source

Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp27-Cys554.[Accession | EHH53214.1]

Source

The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS- PAGE result.

Endotoxin

<1EU per μg by the LAL method.

Purity

> 95% as determined by SDS- PAGE and HPLC

Activity

ELISA Data: Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA..

Bioactivity:Measured by its ability to hydrolyze the 5'-phosphate group from the substrate

 adenosine-5'-monophosphate  (AMP). The specific activity is > 10000 pmol/min/μg.

Formulation

Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100

μg/ml is recommended. Dissolve the lyophilized protein in distilled water.