The mouse model is the most common model for colitis modeling. After the hamster ulcerative colitis model was first prepared by using dextran sulphate sodium (DSS) in 1985, there are a lot of data to prove the etiology, clinical characteristics of DSS colitis model. Symptoms, pathological changes, and treatment response are similar to those of human ulcerative colitis (UC) [1].
Fig 1. Modeling scheme of acute and chronic colitis in mice
1 Acute modeling
1) Sterile drinking water with 3%-5% DSS, and filter with a 0.22 μm membrane, the solution must be colorless or slightly yellowish and clear.
2) Mice are housed in groups, at least 6 mice per cage, and the animals are maintained under standard conditions (with a temperature of 25℃, a 12-h/12-h light/dark cycleand ad libitum access to food and water)
3) The body weight, diarrhea and blood in the stool of the mice were recorded every 24 hours.
4) After rearing for a week, the DSS aqueous solution was replaced with ordinary DSS-free aqueous solution, and the index was finally recorded [2].
2 Chronic modeling
1) Prepare 1%-3% DSS sterile aqueous solution, and the operation is the same as that of acute modeling.
2) Same as acute modeling 2)
3) Same as acute modeling 3)
4) Drink freely for several weeks, and finally replace the DSS aqueous solution with ordinary DSS-free aqueous solution, and finally record the indicators [3].
Results of a successful IBD model
Fig2. HE staining results of DSS acute colitis sections[4]
Links to this resource
- Ohkusa T. Production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and changes in intestinal micro-flora[J]. Nihon Shokakibyo Gakkai Zasshi. 1985;82:1327-1336.
- Xiaona Gao a,b , Wentao Fan a,b , Lei Tan,etc.Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J].J Nutr Biochem 2020 09;83.
- Li Y, Dong J, Xiao H, etc.Gut commensal derived-valeric acid protects against radiation injuries[J].Gut Microbes 2020 07 03;11(4).
- Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.
Successful case for modeling with Yeasen DSS
Yeasen provides high-quality DSS (Cat#60316ES): purity (98%), sulfur content 17-19%, free sulfur <0.2%, supported by a large number of literature data.
Table 1 Construction of different types of enteritis models with DSS
|
Model |
Modeling samples |
Modeling plan |
Modeling results |
Use evaluation |
|
acute colitis |
BALB/c mice, female, 6-8 weeks, 25 g |
3%-5% DSS drink freely for 7 consecutive days |
Day 5 appeared, the length of the colon was shortened, HE staining, and the inflammation was obvious |
molding speed is fast and the time is short. Consistent with the characteristics of acute colitis model |
|
C57BL/6 mice, male, 8 weeks, 20 g |
3%-5% DSS by gavage, continuous administration |
Day 5 occurs, colon shortening, weight loss, blood in the stool, diarrhea |
High mold rate and short duration. Consistent with the characteristics of acute colitis model |
|
|
chronic colitis |
C57BL/6 mouse, male, 8 weeks, 22 g |
1-2% DSS by gavage, continuous administration |
Day40 appears, colon shortening, weight loss, blood in the stool, diarrhea |
High molding rate . Consistent with the characteristics of the chronic colitis model |
|
colon cancer |
C57BL/6 mouse, male, 8 weeks, 21 g |
1%-2% DSS ad libitum for 5 days for 3 weeks |
14 weeks with shortened colon length, weight loss, HE staining, and obvious inflammation |
High molding rate . Consistent with colon cancer model characteristics |
Product order
Hot-selling product only needs 1/3 of the price of M* with same efficiency，and we keep a large stock.
|
Product Name |
Article Number |
Specification |
|
Dextran Sulfate Sodium Salt (DSS), Colitis Grade MW:36000~50000 |
60316ES25/60/76/80 |
25 g/100 g/500 g/1 kg |
|
Azoxymethane (AOM) |
60751ES03/08/10 |
1 mg/5 mg/10 mg |
|
MolPure® Stool DNA Kit |
18820ES08/50/70 |
5 T/50 T/200 T |
|
MolPure® Mag48 Soil/Stool DNA Kit FA |
18528ES48 |
48 T |
|
MolPure®Mag Soil/Stool DNA Kit |
18526ES20/50/70 |
20 T/50 T/200 T |
|
Hematoxylin and Eosin Staining Kit |
60524ES60 |
2×100 mL |
|
Urine Fecal Occult Blood Test Kit |
60403ES60 |
100T |
|
Alcian Blue Periodic Acid-Schiff (AB-PAS) Staining Solution |
60534ES50/60 |
6×50 mL/6×100 mL/ |
Published articles with our reagents
- Zhong D, Jin K, Wang R, ,et al. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26:e2312275. doi: 10.1002/adma.202312275. IF=29.4
- Zhang Y, Tu S, Ji X, Wu J, Meng J, Gao J, Shao X, Shi S, Wang G, Qiu J, Zhang Z, Hua C, Zhang Z, Chen S, Zhang L, Zhu SJ. Dubosiella newyorkensis modulates immune tolerance in colitis via the L-lysine-activated AhR-IDO1-Kyn pathway. Nat Commun. 2024 Feb 13;15(1):1333. doi: 10.1038/s41467-024-45636-x.IF= 16.6
- Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
- Lingjun Tong, Haining Hao, Zhe Zhang,et al.Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota[J].Theranostics.2021; 11(17): 8570-8586 IF=11.556
- Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
- Jingjing Gan, Yuxiao Liu, Lingyu Sun,et al.Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment[J].Applied Materials Today.2021 Dec;25:101231 IF=10.041
- Mengmeng Xu, Ying Kong, Nannan Chen,et al.Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis[J].Frontiers in Immunology.2022; 13: 855645.IF=7.561
- JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
- Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
- Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
- Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885.IF=7.561
- Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
- Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
- Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076