Yeasen Biotech, with years of experience in the field of enzyme raw materials, has been providing high-quality and cost-effective molecular diagnostic PCR raw materials to IVD companies, third-party testing institutions, and research organizations. These include single components such as reverse transcriptase, RNase inhibitor, Taq DNA polymerase, uracil DNA glycosylase (UDG/UNG enzyme), Taq enzyme antibody, dNTPs, as well as system-optimized qPCR/RT-qPCR premix solutions, offering superior performance and stable enzyme raw materials for the development of clinical/non-clinical diagnostic kits.

Hifair® V C58P2 Multiplex One Step RT-qPCR Probe Kit （UDG Plus）

Description

This master mix （Cat#13650ES）is a reagent designed for multiplex quantitative PCR reactions using RNA as the template. During the experimental procedure, reverse transcription and quantitative PCR are carried out in the same reaction tube, streamlining the laboratory workflow and minimizing the risk of contamination.

One-step RT-qPCR reagents integrate the superior performance of reverse transcriptase and hot-start enzymes, complemented by an optimized buffering system, enabling rapid and sensitive detection of single-digit copy templates. Validated by various customer markets including respiratory disease, animal epidemic, and research systems, these reagents can meet the needs of various application directions.

Product advantages

• High sensitivity：The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL；
• Super stability: The product has passed real-time stability testing for 18 months.
• dUP/UDG anti-pollution system：dUTP/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results；
• Support fast program: compatible with fast program, 40min can produce results.

Partial Product Performance Showcase

（1）Sensitivity test

The 13650 stored at -20°C for 18 months (experimental group) and those within the shelf life (control group) were simultaneously tested for the gradient dilution (500 copies/ml, 250 copies/ml) of the pseudovirus SARS-CoV-2, targeting the ORF gene and N gene. Eight replicate wells were tested for each concentration, with a detection rate of 100%. Moreover, there were no significant differences in the amplification curve peak shapes and Ct values.

The 13650 stored at -20°C for 18 months and those within their shelf life. Both groups were tested simultaneously for the SARS-CoV-2 pseudovirus ORF gene and N gene at a concentration of 1000 copies/ml with 20 replicate wells. The detection rate for both was 100%, and there were no significant differences in the shape of the amplification curves or the Ct values.

 （3）stable performance after repeated freeze-thaw cycles

Subjecting the 13650 reagent to 30 and 50 freeze-thaw cycles using dry ice, alongside reagents stored normally at -20°C, and simultaneously testing for the SARS-CoV-2 pseudovirus ORF gene, the results indicated that the performance of the 13650 reagent was unaffected after 50 freeze-thaw cycles.

（4）Compatible with Standard and Rapid Protocols

Using 13650ES, the ORF gene and N gene of SARS-CoV-2 pseudovirus were detected under the same reaction system with both standard and rapid protocols. The results showed that under a 4-plex system, there was no difference in the amplification curve peak shapes and Ct values between the two protocols.