Description
Hieff Unicon™ Pro Universal TaqMan qPCR Master Mix (UDG plus) is a fluorescent quantitative qPCR reagent that uses the company's next-generation antibody method to heat start Taq enzyme. The 2x TaqMan qPCR Mix pre mixed reagent includes reaction buffer and enzyme mix, already containing Mg2+ and dNTP, and adding factors that effectively inhibit non-specific PCR amplification and improve the efficiency of multiple qPCR reactions, which can ensure primer efficiency while conducting up to four reactions. This product contains UDG enzyme, which can effectively prevent the risk of aerosol pollution.
Components
|
Components No. |
Name |
16710ES60 100T |
16710ES80 1,000T |
16710ES92 10,000T |
16710ES94 20,000T |
|
16710 |
2×TaqMan qPCR Mix |
1.25 mL |
12.5 mL |
125 mL |
250 mL |
Specifications
|
Hot Start |
Built-in hot start |
|
Detection method |
Primer-probe detection |
|
PCR method |
qPCR |
|
Polymerase |
Taq DNA polymerase |
|
Type of sample |
DNA |
|
Application equipment |
Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus ™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR. |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.
Instructions
1.Reaction System
|
Components |
Volume (μL) |
Final Concentration |
|
2×TaqMan qPCR mix
|
12.5 |
1× |
|
Primer mix (10 μmol/L) |
x |
0.1-0.5 μmol/L |
|
Probe mix (10 μmol/L) |
x |
50-250 nmol/L |
|
Rox reference dye |
0.5 |
1× |
|
Template DNA/cDNA |
1-10 |
- |
|
ddH2O |
up to 25 |
- |
*The primer concentration can be adjusted appropriately according to the experimental requirements.
2. Reaction program
|
Cycle step |
Temp. |
Time |
Cycles |
|
UDG treatment |
37℃ |
5 min |
1 |
|
Initial denaturation |
95℃ |
5 min |
1 |
|
Denaturation |
95℃ |
15 s |
45 |
|
Annealing/Extension |
60℃ |
30 s |
*The annealing / extension temperature can be adjusted appropriately according to the experimental requirements.
Notes
Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！