Molecular biology

The Reasonable Range of Ct Values in qPCR Experiments

The Reasonable Range of Ct Values in qPCR Experiments

qPCR
Ct value is a crucial outcome representation in fluorescent quantitative PCR. It is used to calculate differences in gene expression levels or gene...
The Reasonable Range of Ct Values in qPCR Experiments

The Reasonable Range of Ct Values in qPCR Experiments

qPCR
Ct value is a crucial outcome representation in fluorescent quantitative PCR. It is used to calculate differences in gene expression levels or gene...
Simplify the complex! Let me guide you through qPCR data analysis with ease

Simplify the complex! Let me guide you through qPCR data analysis with ease

qPCR
Fluorescent quantitative PCR (qPCR) is a commonly used technique in laboratories, which can be applied to gene expression analysis, genotyping, pat...
Work along both lines, miRNA expression analysis made easier

Work along both lines, miRNA expression analysis made easier

Work along both lines, miRNA expression analysis made easier The length of mature miRNA is only about 20nt, usually, the forward primer will cover...
Direct PCR, No Extraction

Direct PCR, No Extraction

Direct PCR, No Extraction Direct PCR is a reaction that directly uses blood, animal or plant tissue, etc., for amplification without steps for nuc...
What to do when the qPCR results are weird?

What to do when the qPCR results are weird?

What to do when the qPCR results are weird? qPCR experiments can feel easy and sometimes difficult. After the primer design is completed, qPCR sam...