PCR (Polymerase Chain Reaction), a basic experimental technology that an experimenter cannot do without. Since its birth, the technology, after more than 30 years of development, has emerged in a variety of application scenarios. However, PCR products on the market emerge in endlessly, but not necessarily to meet the needs of everyone. Did the following scenarios appear:
Question 1: I have increased the extension time to more than 1 min / kb, the annealing temperature is also the optimal temperature of the primer, or can not expand the band?
Question 2: I can’t eat on time again today! The cloning plate is covered with bacterial colonies, and the PCR amplification identification time is too long, it takes half a day to run once, and the amount of product expansion is still small, alas...
Question 3: This plant sample was finally collected from the mountain. For this plant, which PCR reagent can expand the band I want at a time?
Question 4: Tomorrow will be handed in the group will report, the experiment has not progressed, but it is too difficult to use E. coli colony / liquid to expand the fragment, accidentally expand the non-specific band, come to help me!
In the face of the above phenomenon, do not worry, YEASEN this to solve! YEASEN new and upgraded rapid PCR premixed solution (10167ES) can not only meet the needs of your colony PCR, but also provide rapid amplification reaction, saving you precious time and retaining precious samples!
2×HieffTM Ultra-Rapid II HotStart PCR Master Mix
Product Advantage
More faster: Colony PCR up to 1 sec / kb.
More general: GC up to 70%, length up to 10 kb long.
More reliable: Hundreds of fragments of more than ten species.
More efficient: High specificity, with a detection rate of up to 100%.
More stable: The effect period is as long as 2 years, repeated freezing and thawing to take the worry.
Application Scenarios
- Colony PCR
- Rapid PCR
- Genotype identification
Performance Display
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Rapid and Efficient Amplification of Colonies
Fig. 1 E. coli colony limit extension time amplification test, the extension efficiency of 3 kb fragments amplified by 10167ES can reach 1 sec/kb, 6 kb fragment amplification efficiency can reach 3 sec/kb, and 6-10 kb fragment amplification efficiency can reach 5 sec/kb. M: 10,000 DNA Marker (10505ES).
Fig. 2 10167ES amplifies 1-10 kb Agrobacterium colonies, with a detection rate of 100%, high yield, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.
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Rapid and Efficient Amplification of Long Fragments + High GC Bacterial Liquid
Fig. 3 Long fragments + high GC bacterial liquid amplification, the results show that 10167ES has a high amplification yield, a detection rate of 100%, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.
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Rapid and Efficient Amplification of Genomic DNA
Fig. 4 10167ES amplifies 1-10 kb mouse gDNA, with high amplification yield, a detection rate of 100%, and better performance than competitors. M: 10,000 DNA Marker (10505ES). 10167ES amplification speed is 10 sec/kb, while the competitor's amplification speed is 15 sec/kb.
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Rapid and Efficient Amplification of λDNA
Fig. 5 10167ES amplifies 5-15 kb λDNA, with an extension efficiency of 1 sec/kb for fragments within 5-10 kb, and 3 sec/kb for 15 kb fragments. M: 10,000 DNA Marker (10505ES).
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Reagent Stability, No Worries with Repeated Freeze-Thaw Cycles
Fig. 6 10167ES remains stable after 100 freeze-thaw cycles, still capable of amplifying 5 kb λDNA and 5 kb human gDNA. M: 10,000 DNA Marker (10505ES).
Fig. 7 10167ES remains stable after being heated at 37°C for 14 days, still capable of amplifying E. coli, Agrobacterium, and yeast colonies. M: 10,000 DNA Marker (10505ES).
Customer Case
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High-Efficiency Amplification of Low GC Fragments
Experimental Information: Target fragment 1,100 bp
Fig. 8 10167 amplifies 1.1 kb E. coli colonies, gene source: Methanobrevibacter smithii (GC 30%), high detection rate, high band brightness, better performance than competitors, correct sequencing results. Extension time is 15 sec/kb. Number of cycles: 30 cycles.
Customer Review: The results with YEASEN were better, with higher band brightness and fewer side bands!
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Rapid Amplification of Colonies
Experimental Information: Target fragment 1,200-1,300 bp
Fig. 9 10167ES amplifies 1.2-1.3 kb E. coli colonies, fast amplification speed, high band brightness, better performance than competitors, correct sequencing results. Extension time for 10167ES is 3-20 sec/kb, for competitors, it's 20 sec/kb. Number of cycles: 33 cycles.
Customer Review: The sequencing signal was good, and it could be detected. If the time was 1 sec/kb, I think it's very fast, and the brightness of the gel is quite bright, the effect is good!
Experimental Information: Target Fragment 3,000 bp
Fig. 10 Amplification of a 3 kb (GC 38%) Bacillus cereus colony with 10167ES, with an ultra-fast amplification speed of up to 1 sec/kb, resulting in high yield and superior performance compared to competitors. The number of cycles was 35.
Customer Review: The effect is excellent, the PCR bands are very bright, and amplification at 1 sec/kb is more than sufficient, without the need to extend the time. This saved over an hour compared to other brands, which can improve experimental efficiency!
Experimental Information: Target fragment 2,500 bp
Fig. 11 10167ES amplifies 2.5 kb Agrobacterium colonies, high amplification efficiency, 100% detection rate. Extension time is 10 sec/kb. Number of cycles: 35 cycles.
Customer Review: It was amplified in one go, it's easy to use!
Experimental information: the target fragment is 2,375 bp
Fig. 12 10167ES Amplified 2,375 bp of E. coli solution, amplified as fast as 1 sec / kb, with high efficiency and better performance than competing products. The extension time of 10167ES is 1 sec / kb, and competitor is 15 sec / kb. Cynumber 34 cycles.
Customer Review: The amplification speed is faster, the E. coli liquid template strip looks brighter!
Ordering information
|
Application |
Name |
No |
Specifications |
|
Rapid PCR upgrade, can be colony PCR and suitable for complex template amplification |
2×HieffTM Ultra-Rapid II HotStart PCR Master Mix |
1 mL/5×1 mL |
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|
|
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