Targeted next generation sequencing (tNGS) products continue to be launched, officially opening the era of pathogen mNGS 2.0. By targeting the amplification of specific pathogenic microorganisms, tNGS not only enhances detection sensitivity but also eliminates interference from host nucleic acids. Compared with mNGS, it significantly improves the detection sensitivity of target pathogens and the detection performance of drug resistance genes. Moreover, it can handle both DNA and RNA workflows, truly achieving the broad coverage, ultra-high sensitivity, and cost-effectiveness required in clinical settings.
tNGS Detection Process
Pathogen tNGS protocol
Protocol 1 Pathogen DNA&RNA Co-extraction
MolPureTM Magnetic Pathogen DNA/RNA Kit（Cat#18306）
Good sample compatibility: compatible with swabs, whole blood, plasma, sputum, bronchoalveolar lavage fluid, cerebrospinal fluid, and other samples. Easy and quick operation: Single sample extraction takes only 40 minutes. 、Broad applicability of pathogenic bacteria: A combination of chemical and mechanical methods ensures that the purified nucleic acids (DNA/RNA) include viruses, mycoplasmas, bacteria, fungi, and other microorganisms.
Protocol 2 Universal multiplex PCR premix
4×HieffTM Multiplex PCR Master Mix（Cat#12948）
Easy operation: High multiplication premix contains all components except primers and DNA templates.
Excellent performance: High uniformity, high specificity and high sensitivity, low background residue;
Wide compatibility: Compatible with GC content of 25% -70%;
Compatible with various application scenarios: tNGS pathogenic microbial detection, environmental microbial identification, and food safety testing.
Protocol 3 UDG Antipollution Multiplex PCR Premix
4×HieffTM Multiplex PCR Master Mix(UDG plus) （Cat#12947）
Significant anti-pollution effect: UDF Anti-Contamination Technology for Easy Decontamination;
Compatible with reverse transcription system: no purification is required after reverse transcription, and multiple amplification can be performed directly.
Strict background bacteria control: Clean laboratory, professional personnel, and strict quality control.
Excellent amplification performance: high detection rate, excellent specificity and good uniformity.
Protocol 4 One-step RT-mPCR premix
4×HieffTM Multiplex RT-PCR Master Mix（Cat#12950）
Easy operation: reverse transcription and multiplex PCR are completed in one step, simplifying the process and saving time;
Excellent amplification performance: high detection rate, excellent specificity and good uniformity;
Strict background bacteria control: Clean laboratory, professional personnel, and strict quality control.
Ultimate delivery and high cost-performance ratio have always been the voice of patients with infectious diseases. Multiple DNA & RNA library construction kits meet the diverse needs of customers, facilitating more accurate and faster detection of pathogen tNGS probe capture products!
tNGS Guideline
|
Category |
Product Name |
Item Number |
Note |
|
Reverse |
Hieff NGSTM 1st Strand Synthesis Kit |
12249ES |
Common |
|
Hieff NGSTM 1st Strand Synthesis Kit |
12946ES |
Fast |
|
|
Hieff NGSTM 1st Strand Synthesis Mix |
12958ES |
Master Mix |
|
|
Multiplex |
4×HieffTM Multiplex PCR Master Mix |
12948ES |
- |
|
4×HieffTM Multiplex PCR Master Mix(UDG plus) |
12947ES |
Anti-pollution |
|
|
One-step |
HieffTM Multiplex RT-PCR Kit |
12950ES |
- |