Nucleic acid serves as the foundation of all molecular biology research. Moreover, the quality of extracted nucleic acid is also one of the key factors determining the success or failure of downstream experiments. When developing nucleic acid extraction reagents, in addition to conventional chemical components, some small molecule nucleic acids or enzymes can greatly improve the extraction efficiency or remove the interference of non-target substances and become important components in nucleic acid extraction.

On auxiliary substances for nucleic acid precipitation

Carrier RNA

1. Concept and functionof Carrier RNA

Carrier RNA, also known as nucleic acid precipitation aid in Chinese. In the nucleic acid extraction process, the centrifuge tubes used for extraction are mostly made of polypropylene. There is static electricity on the tube wall, which will adsorb nucleic acids. Using Carrier RNA in the extraction and purification process can help nucleic acids remove the electrostatic effect, ensure that nucleic acids can be well adsorbed onto the purification column, improve the elution efficiency, and thus achieve the recovery of nucleic acids in the sample as much as possible.

2. Common types ofCarrier RNA

As an auxiliary substance for nucleic acid precipitation, Carrier RNA has multiple types, such as PolyA potassium salt, total E. coli RNA, total yeast RNA, tRNA, etc.

3. Common Problems in Useof Carrier RNA

Can Carrier RNA still be used after being placed at room temperature for a period of time? Does the degradation of Carrier RNA affect its use? Does Carrier RNA affect downstream experiments?
Carrier RNA is dissolved in an RNA protectant. The liquid Carrier RNA has little impact when placed at 37°C or room temperature for a short period (within 7 days). Essentially, Carrier RNA is a fragment in the range of 500 nt to 1000 nt. Slight degradation does not affect normal use. For downstream applications such as fluorescent quantitative PCR, Carrier RNA has no effect on the Ct value. For downstream applications such as NGS, it can be added according to actual needs.

4. Product Introductionof Carrier RNA

Yeasen's Carrier RNA is RNA derived from yeast. There is no residual DNase or RNase enzymes, and the residual content of protein and DNA is extremely low. It can be used in experiments such as viral nucleic acid extraction and trace DNA extraction. The extracted nucleic acids can be used in subsequent experiments such as RT-PCR and RT-qPCR. The product is available in liquid and lyophilized powder formulations for convenient direct use, long-distance transportation, and storage.

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
Carrier RNA, 6 μg/μL	19701ES	1 mL / 5 mL / 100 mL	Stored at -25 to -15 °C. Shelf life is 2 years.
Carrier RNA Powder, 216 μg/tube	19703ES	1 tube / 100 tube / 1000 tube	Stored at room temperature.Shelf life is 2 years.
Carrier RNA Powder, 310 μg/tube	19704ES	1 tube / 100 tube / 1000 tube	Stored at room temperature.Shelf life is 2 years.

Linear acrylamide solution

1. Concept and function of linear acrylamide solution

Linear acrylamide, with the English name Linear Acrylamide (Linear Polyacrylamide, abbreviated as LPA), is a coprecipitant that helps precipitate nucleic acids during the nucleic acid purification process.

2. Characteristics of linear acrylamide solution

It is a neutral carrier and of non-biological origin. Therefore, it does not contain potential nucleic acid or nuclease contamination.

It will not interfere with A260/A280 readings and will not inhibit polymerase and restriction endonuclease activities.

It is compatible with other subsequent molecular biology applications such as PCR and enzymatic digestion.

It can assist in precipitating picogram-level or above DNA and RNA (including Oligo). The recoverable fragment length is above 20 nucleotides, and both single-stranded and double-stranded nucleic acids are applicable.

3. Product Introduction

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
Linear Acrylamide Solution(5 mg/mL)	10408ES	1 mL	Stored at 4 °C. Shelf life is 3 years.

Nucleic acid extraction auxiliary enzyme

RNase A

1. Concept and function of RNase A

Ribonuclease A (RNase A), as an endoribonuclease, specifically degrades cytosine (C) or uracil (U) residues on single-stranded RNA. The most common application of ribonuclease A (RNase A) is to remove RNA during the preparation of plasmid DNA or genomic DNA. In addition, this product can also be used in molecular biology experiments such as RNase protection assays and RNA sequence analysis.

2. Characteristics of RNase A

RNase A is derived from Bovine pancreas. There is no contamination of endo- and exonuclease deoxyribonuclease. It can be used directly without preheating and is resistant to high temperature environments.

3. Product Introduction

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
RNase A(100 mg/mL)	10406ES	1 mL	Stored at -20°C. Shelf life is 2 years.
RNase A(10 mg/mL)	10405ES	1mL	Stored at room temperature.Shelf life is 1 years.
Ribonuclease A(RNase A),from bovine pancreas	10407ES	100mg/1g	Stored at room temperature.Shelf life is 2 years.

Deoxyribonuclease I (DNase I)

Deoxyribonuclease I (DNase I), an endonuclease found in various cells and tissues, targets and cleaves the phosphodiester bond adjacent to pyrimidines, producing polynucleotides with a phosphate group at the 5' end and a hydroxyl group at the 3' end. The average minimum digestion product is a tetra-nucleotide polymer. DNase can catalyze various forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (whose cleavage rate is affected by histones). In molecular biology experiments, it is often used to remove DNA from proteins or to introduce nicks into DNA to insert labeled bases into DNA.

2.Characteristics of DNase I

The optimal working range of DNase I is pH 7-8, and its activity depends on Ca²⁺ and can be activated by divalent metal ions such as Co²⁺, Mn²⁺, Zn²⁺, etc. 5 mM Ca²⁺ can protect the enzyme from being hydrolyzed. In the presence of Mg²⁺, this enzyme can randomly recognize and cut any site on either strand of DNA; while in the presence of Mn²⁺, it can recognize both strands of DNA simultaneously and cut at almost the same site.

3.Product Introduction

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
Deoxyribonuclease I(DNase I) from bovine pancreas	10607ES	15 KU/10×15KU	Stored at -20°C. Shelf life is 2 years.
Deoxyribonuclease I(DNase I) from bovine pancreas	10608ES	25 mg/100mg/1g	Stored at -20°C. Shelf life is 2 years.

Proteinase K

1.Concept and function of Proteinase K

Proteinase K (Proteinase K) is a serine protease with a wide range of cleavage activities. Its relative molecular weight is about 29.3 kDa and it can cleave the carboxyl-terminal peptide bonds of aliphatic amino acids and aromatic amino acids. Proteinase K has a wide range of applications and can be used in experiments such as preparing chromosomal DNA for pulsed-field gel electrophoresis, Western blotting, and removing nucleases in DNA and RNA preparations. Denaturing agents such as SDS (1%) can increase its activity.

2.Characteristics of Proteinase K

Derived from a genetically recombinant yeast strain.

Free of RNase and DNase.

Active and stable in urea and SDS.

Active in a wide pH range (pH 4.0 - 12.0), and the optimal pH range is pH 7.5 - 9.0.

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
Proteinase K	10401ES	100mg/1g/5 g/100g	Stored at 4°C. Shelf life is 3 years.
Proteinase K Solutio(20 mg/mL)	10412ES	1mL/10mL/25mL/500mL	Stored at 4°C. Shelf life is 2 years.
Proteinase K Solution(20 mg/mL)	10410ES	1mL/10mL/25mL/500mL	Stored at 4°C. Shelf life is 2 years.

RNA stabilizer

RNA stabilizer solution for animal and plant tissues

1.Function of RNA stabilizer solution for animal and plant tissues

RNases are widely present in the environment and are very likely to degrade the RNA in freshly collected samples of animal and plant tissues, blood, body fluids, etc. The RNAsafe tissue stabilizer for animal and plant tissues (RNAsafe Tissue Stabilizer) is specially developed to stabilize and protect the RNA in collected samples. As a non-toxic solution, it can quickly penetrate tissues and inactivate endogenous RNases.

2.Characteristics of RNA stabilizer solution for animal and plant tissues

Extremely easy to operate: Just cut fresh tissues into tissue blocks of appropriate size (≤0.5 cm) and immerse them in 5 to 10 times the volume of RNAsafe.

Long stabilization period: Tissues/cells infiltrated with RNAsafe can be stored at 37°C for 1 day, at room temperature for 1 week, at 4°C for 4 weeks, and for long-term storage at -20°C/-80°C.

Wide downstream compatibility: It is compatible with almost all RNA isolation methods, such as HieffTM Total RNA Extraction Reagent (catalogue number: 10606ES60), and almost all commercial RNA extraction kits, including centrifugal column extraction method and magnetic bead purification method.

Wide application of materials: It can be used for the preservation of various animal and plant tissues (such as brain, heart, liver, pancreas, kidney, spleen, testis, muscle, stems and leaves of plants, etc.), and also for yeast, tissue culture cells, etc.

3.Product Introduction

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
RNAsafe Tissue Stabilizer RNAsafe	10604ES60	100 mL	Stored at room temperature.Shelf life is 5 years.

Related products

Product Name	Product Number	Specification	Storage Conditions and Shelf Life
Lysozyme	10402ES	5/50 g	Stored at -20°C. Shelf life is 3 years.
Lyticase (10 U/μL)	10403ES	1500/3000/10000 U	Stored at -20°C.
Snailase	10404ES	5/10 g	Stored at 2-8°C. Shelf life is 2 years.
RNAsafe-ICE	10605ES	100 mL	Stored at room temperature.Shelf life is 2 years.