Description
Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.
Applications
- Genomic DNA extraction
- Digestion and removal of the enzyme
Specifications
| Source | Yeast |
| Specific Activity | ≥30 U/mg |
| Unit Definition | One unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5. |
| Purification | Ni column affinity purification |
| Reaction Condition | The reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃. |
| Inactivation method | The reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min. |
| Activity Range | It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0. |
| RNase and DNase | None |
Components
| Components No. | Name | 10401ES60 | 10401ES80 | 10401ES90 | 10401ES98 |
| 10401 | Proteinase K | 100 mg | 1 g | 5 g | 100 g |
Shipping and Storage
The product is shipped with ice packs and can be stored at 2℃ ~ 8℃ for three years.
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