ECL Series Luminescence Kit — Light up your imprinted membrane
Product description
ECL reagents, also known as horseradish peroxidase (HRP) substrates, are currently the most sensitive reagents for Western blotting detection. At present, common ECL reagents on the market often have some problems such as easy quenching, high background, instability and low abundance protein difficult to detect, which affect the final experimental results.
According to the current situation, YEASEN concentrate excellent scientific researchers in the field of immune detection, on the basis of strict control of luminol raw materials, and constantly improve the reinforcer and stabilizer formula, according to different needs to develop 4 kinds of ECL reagents, including Enhanced, Super, SuperDura and MaxiSignal products. It can not only meet the requirements of the experimenter for the detection of the expression of the target protein from low abundance to high abundance, but also improve the luminescence intensity without affecting the experimental conditions, so that you can get a clearer image.
Excellent sensitivity — can detect pick to low fick grade antigens
Higher signal-to-noise ratio — precise luminescent substrate reduces background
Greatly save antibody — Optimized substrate system, higher antibody binding force
Excellent cost performance — higher performance, lower price
Excellent stability — new oxidant, stable storage at 4℃ for one year
Product principle
The core principle of ECL reagent detection is oxidation reaction: Luminol as the main ingredient of luminous substrate, in alkaline conditions,catalyzed by horseradish peroxidase (HRP), it is oxidized by H2O2 to produce the excited intermediate of 3-amino-phthalic acid. When it returns to the ground state, it emits a photon with a maximum emission wavelength of 425 nm. The photon signal can be captured by X-ray film or CCD imager.
Selection guide
At present, YEASEN has 4 kinds of ECL products, each with its own characteristics, which can fully meet users' requirements for western blotting applications.
So, which product should you choose for your experiment?
Table 1. ECL reagent selection guide
|
YEASEN |
Enhanced ECL |
Super ECL |
SuperDura |
MaxiSignal |
|
Applicable samples |
Target abundance is higher, conventional protein samples |
Target abundance is low, limited protein samples |
Target abundance is lower,limited protein samples |
Target abundance is very low, precious protein samples |
|
Detection sensitivity |
medium pick-grade |
low pick-grade |
medium fick-grade |
low fick-grade |
|
Signal duration |
< 2 h |
< 12 h |
< 24 h |
< 8 h |
|
Applicable detection system |
All reagents are suitable for X - ray film, CCD imager |
|||
|
Dilution ratio of primary antibody |
1:1000-1:4000 |
1:1000-1:10,000 |
1:1000-1:50,000 |
1:5000-1:100,000 |
|
Dilution ratio of secondary antibody |
1:1000-1:4000 |
1:2000-1:10,000 |
1:50,000-1:250,000 |
1:100,000-1:500,000 |
|
Recommend membrane |
NC membrane |
NC membrane or PVDF membrane |
NC membrane or PVDF membrane |
NC membrane or PVDF membrane |
|
Storage stability |
room temperature for one year |
room temperature for one year |
room temperature for one year |
4℃ for one year, room temperature for half of a year |
|
advantage |
The signal is strongest at the same level of sensitivity |
The most cost-effective, suitable for most WB experiments |
Maximum signal duration, ideal for multiple exposures |
Excellent sensitivity, extremely suitable for trace protein detection |
[Note] : The recommended dilution concentration of antibody is that of antibody storage solution when the concentration is 1 mg/mL.
Data show
Yeasen ECL reagent has better use effect than other brands of the same level of products.
Figure 1. Comparison of detection effect between Yeasen Super substrate and Pico substrate of the same level of other brands
Figure 2. Comparison of detection effect between Yeasen Maxisignal substrate and West Femto of the same level substrate of other brands
Figure 3. Comparison of detection effect between Yesen SuperDura substrate and West dura of the same level substrate of other brands
Citations & References
[1] Wang Z, Liu C, Zhu D, et al. Untangling the co-effects of oriented nanotopography and sustained anticoagulation in a biomimetic intima on neovessel remodeling[J]. Biomaterials. 2020;231:119654. (IF:10.317)
[2] Shang J M , Ma S R, Zang C X, et al. Gut microbiota mediates the absorption of FLZ, a new drug for Parkinson’s disease treatment[J]. Acta Pharmaceutica Sinica B 2021;11(5):1213-1226. (IF:11.4)
Ordering Products
|
Product Name |
Cat# |
Specifications |
|
Super ECL Detection Reagent |
36208ES60/76 |
100 mL/500 mL |
|
Enhanced ECL Chemiluminescent Substrate Kit |
36222ES60/76 |
100 mL/500 mL |
|
36223ES10/60/70/76 |
10 mL/100 mL/200 mL/500 mL |
|
|
36224ES10/60/70/76 |
10 mL/100 mL/200 mL/500 mL |