Description
Super ECL Detection Reagent is designed to detect antibodies and associated antigens directly or indirectly labeled with horseradish peroxidase (HRP). The principle of Super ECL Detection Reagent is that, proteins or nucleic acids were transferred to the imprinted membrane after electrophoresis, and the target proteins on the membrane were bound by primary antibody and secondary antibody labeled with HRP, or the nucleic acids on the membrane were bound directly or indirectly by probes labeled with HRP. After washing the membrane, the ECL working solution prepared by the product was used to incubate the membrane at room temperature for several minutes. The imprinted membrane was wrapped with plastic wrap and fixed to the X-ray exposure Cassette. Then the X-ray film is pressed on the membrane in a darkroom and exposed for several seconds to several hours. After development and fixing, protein or nucleic acid bands can be clearly displayed on the X-ray film. This kit has a unique luminescent substrate system, Super ECL Detection Reagent is the most sensitive commercial fluorescent ECL detection reagent at present.
Features
- High sensitivity and high signal-to-noise ratio, it can detect low pick-grade antigens.
- Shine quickly and brightly, the imprinted film band can be observed under fluorescent lamp.
- Long duration of luminescence, fluorescence can make X-ray film sensitive for more than 5 hours, especially suitable for detecting trace protein or nucleic acid.
- Higher dilution ratio of antibody (1:2000 ~ 1:10000) can be used to greatly save antibody.
Applications
- Chemiluminescence ELISA
- Western Blot
- Dot Blot-DNA/RNA
- Southern blot-DNA
- Northern blot-RNA
Specifications
| Recommended Antibody Concentrations | primary antibody (liquid storage concentration 1mg/mL) : 1:1000-1:10000 secondary antibody (liquid storage concentration 1mg/mL) : 1:2000-1:10000 |
| Signal Duration | < 12 h |
| Substrate Properties | Chemical Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) Substrate |
| Sensitivity | low pick-grade antigens |
Components
| Components No. | Name | 36208ES60 (100 mL) | 36208ES76 (500 mL) |
| 36208-A | Super ECL Detection Reagent-Reagent A | 50 mL | 250 mL |
| 36208-B | Super ECL Detection Reagent-Reagent B | 50 mL | 250 mL |
Shipping and Storage
The product is shipped with ice pack and can be stored at 2℃ ~ 8℃ for one year away from light.
Figures
Figure 1 Comparison of detection effect between Yeasen Super substrate and Pico substrate of the same level of other brands
Yeasen ECL reagent has better use effect than other brands of the same level of products
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