Adeno-associated viruses (AAV) and lentiviruses (LV) are widely used in cell and gene therapy due to their high safety and efficacy profiles. These vectors are typically produced on a large scale in HEK293 cells using polyethylenimine (PEI)-mediated transient transfection methods. YEASEN Biotech, leveraging its strong R&D capabilities, offers a range of high-quality PEI-based transfection reagents, with specific modifications to PEI to enhance its performance in particular application scenarios. To address the challenge of producing sufficient quantities of AAV for clinical development, YEASEN Biotech has developed a novel transfection reagent, Ultra PEI-AAV, specifically designed for large-scale AAV production in suspension systems and suitable for use in large bioreactors.
Ultra PEI-AAV- Suspended System AAV Virus Production Transfection Reagent.
Economical and efficient: In the suspension system, higher AAV virus titers can be produced, and higher virus yields can still be achieved with less DNA.
Operationally convenient: The complex volume can be reduced to 1%, and the transfection complex stability can last up to 6 hours, meeting the needs of large-scale production.
Easy to scale up:Â Offers both research-grade and GMP-grade products, meeting the needs from small-scale processes to large-scale production.
Broad applicability: Capable of producing higher virus titers for a variety of AAV serotypes.
Achieves extremely high virus titers for various AAV serotypes.
Ultra PEI-AAV transfection reagent, compared to PEI and other competitors, can significantly increase AAV production when producing AAV2/AAV5/AAV8/AAV9 in suspended 293F cells, with virus yields markedly higher than those of competitors; it also has a high transfection efficiency for different serotypes.
Higher AAV titers with less DNA usage.
Compared to competitors, Ultra PEI-AAV transfection reagent can enhance virus production while using less DNA, significantly reducing production costs in large-scale manufacturing.
Provides flexibility for large-scale production.
The transfection complex volume can be reduced to 1%, and it has a stability of up to 6 hours. These characteristics together provide great convenience and cost-effectiveness for the large-scale preparation of AAV viral vectors.
YEASEN Biotech, as a global leader in the supply of biopharmaceutical raw materials, is committed to providing R&D and GMP-grade PEI transfection reagents. Our products, known for their exceptional high transfection efficiency and low usage, help customers significantly increase yield while reducing production costs, achieving cost reduction and efficiency enhancement. Relying on rich industry experience and strict GMP production standards, Our products have successfully supported many projects to complete IND project declarations. Production strictly adheres to the ISO 13485 quality system, ensuring the consistency and reliability of our products. At the same time, we actively collaborate with customers to provide project submission document support and cooperate in completing audit processes, offering comprehensive and efficient services.
Product List
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Product Name |
Product Specifications |
Product number |
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1 mL /10 mL /100mL |
40823ES03/10/60 |
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Hieff Trans® Ultra PEI-AAV Transfection Reagent-GMP |
10 mL /100 mL/1L |
40824ES10/60/80 |