Description
This product is specifically developed for the large-scale production of AAV in suspension systems. It is an optimized and modified linear polyethylenimine (Polyethylenimine Linear, PEI). The product is synthesized purely through chemical processes, free from animal-derived components, and has low cytotoxicity. It also offers broad applicability across various AAV serotypes. With high DNA loading capacity, a low transfection complex ratio, and high complex stability, this product facilitates large-scale AAV production.
The product is manufactured in accordance with GMP requirements and is provided in the form of a sterile liquid.
Features
Significant Increase in Virus Titer: The product achieves a substantial increase in virus yield, with approximately a 2-fold increase in virus titer for multiple serotypes, including AAV2, AAV5, AAV8, and AAV9.
Reduced Usage Cost: By reducing the amount of transfection reagent by 50% while maintaining high yield, the product effectively lowers production costs.
Applications
Production of AAV Viral Vectors
Specifications
|
Form |
liquid |
|
Properties |
Polyethylenimine Linear (PEI) |
|
Virus Type |
AAV |
Components
|
Components No. |
Name |
40824ES10 |
40824ES60 |
40824ES80 |
|
40824 |
Hieff TransTM Ultra PEI-AAV Transfection Reagent-GMP |
10 mL |
100 mL |
1 L |
Shipping and Storage
Hieff TransTM Ultra PEI-AAV Transfection Reagent-GMP products should be stored at 2-8℃ for 2 year.
Figures
Figure1. AAV2, AAV5, AAV8, and AAV9 were produced in suspension 293F cells, with a DNA dosage of 1 µg per million cells. The virus was harvested 72 hours post-transfection, and the viral supernatant was analyzed.
Figure2. AAV9 was produced in suspension 293F cells with different input of Ultra-PEI (Left, Plasmids input: 0.5 μg) or Plasmids (Right, Ultra-PEI input 0.6 μL) dosages per million cells. The virus was harvested 72 hours post-transfection.
Documents:
Safety Data Sheet
Manuals
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.