Source

Recombinant Human IL-22R alpha 1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains His16-Thr228.[Uniprot| Q8N6P7]

Molecular Weight

The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE;    > 95% as determined by HPLC

活性（Activity）

ELISA Data 1:Immobilized Human IL-22R alpha 1, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-22R alpha 1 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.

ELISA Data 2:Immobilized Human IL-22, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-22R alpha 1, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.