Source

Biotinylated Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A).[Accession | P29460(IL-12B)&P29459(IL-12A)]

Molecular Weight

The protein has a predicted MW of 34.7(IL-12B) kDa / 25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Anti-IL-12 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 13.0ng/ml determined by ELISA.  

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.