شرح
BACE-1 is an aspartic protease and an integral membrane protein (1-5). BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5-5.5), consistent with its proposed function in an acidic intracellular compartment.
Specifications
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Synonyms |
Beta-secretase 1; ASP2; Asp 2; BACE1; BACE; FLJ90568; HSPC104; KIAA1149; Memapsin-2; BACE-1; EC 3.4.23; EC 3.4.23.46;β-secretase 1 |
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Uniprot No. |
Q68D85.1 |
|
Source |
Recombinant Human BACE-1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Thr22-Thr457. |
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Molecular Weight |
Approximately 49.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
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