شرح
CD40 Ligand, also known as TNFSF, a type II membrane protein with homology to TNF, is transiently expressed on activated T cells and known to be important for B cell Ig production and for activation and differentiation of monocytes and dendritic cells. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region . It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses.
Specifications
|
Synonyms |
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
|
Uniprot No. |
P29965 |
|
Source |
Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 Cells with monomeric hFc tag at the N-terminal. It contains Met113-Leu261. |
|
Molecular Weight |
Approximately 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13μg/ml determined by ELISA. Blocking Data：Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
پرداخت و امنیت
اطلاعات پرداخت شما با اطمینان پردازش می شود. ما جزئیات کارت اعتباری را ذخیره نمی کنیم و به اطلاعات کارت اعتباری شما دسترسی نداریم.
تحقیق
شما همچنین ممکن است دوست داشته باشید
پرسش
این محصول فقط برای اهداف تحقیقاتی است و برای استفاده درمانی یا تشخیصی در انسان یا حیوانات در نظر گرفته نشده است. محصولات و محتوا توسط پتنت ها، علائم تجاری، و حق چاپ متعلق به Yeasen Biotechnology محافظت می شوند. نمادهای علامت تجاری نشان دهنده کشور مبدا هستند، نه لزوما ثبت در همه مناطق.
برخی از برنامه‌های کاربردی ممکن است به حقوق مالکیت معنوی شخص ثالث دیگری نیاز داشته باشند.
Yeasen به علم اخلاق اختصاص داده شده است و معتقد است که تحقیقات ما باید به سؤالات مهم و در عین حال اطمینان از ایمنی و استانداردهای اخلاقی بپردازد.