Beschrijving
|
Product Name |
Cat# |
Size |
|
Recombinant Human IL-10 Protein |
90109ES05 |
2 μg |
|
90109ES10 |
10 μg |
|
|
90109ES50 |
50 μg |
|
|
90109ES60 |
100 μg |
|
|
90109ES76 |
500 μg |
Interleukin 10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types, including macrophages, monocytes, T cells, B cells, and keratin-forming cells.IL-10 inhibits the expression of pro-inflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune responses.
This recombinant human IL-10 is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.
Specification
|
Synonyms |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF；IL10 |
|
Source |
E.coli-derived human IL-10 protein, Ser19-Asn178. |
|
Accession |
P22301 |
|
Molecular Weight |
Approximately 18.6 kDa. |
|
AA Sequence |
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 95%, SDS-PAGE and HPLC detection. |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, 1 year expiration date after receipt.
After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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FAQ
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