Beschrijving
Specifications
|
Source |
Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminal. It contains Met113-Leu261.[Accession | P29965]. |
|
Molecular Weight |
The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on SDS- PAGE result. |
|
Endotoxin |
<1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS- PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
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