Beschrijving
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a 130-155 kDa member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Within ECD human Flt-3 shares 85% and 82% aa sequence identity with mouse and rat Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations.
Specifications
|
Synonyms |
CD135, FLK2, STK-1, FLT-3 |
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Uniprot No. |
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|
Source |
Recombinant Human FLT3 / Flk-2 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Asn27-Asn541. |
|
Molecular Weight |
Approximately 61.2 kDa. Due to glycosylation, the protein migrates to 80-105 kDa based on Tris-Bis PAGE result. |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human FLT3, His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 0.1 μg/mL determined by ELISA. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transport with ice packs. Store at -20℃ to -80℃, valid for one year.
Once reconstituted, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2-8°C for 2-7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
Tris-Bis PAGE
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