Beschrijving
Specifications
|
Source |
Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp27-Cys554.[Accession | EHH53214.1] |
|
Source |
The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS- PAGE result. |
|
Endotoxin |
<1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS- PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.. Bioactivity:Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is > 10000 pmol/min/μg. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
Betaling en beveiliging
Uw betalingsinformatie wordt veilig verwerkt. We slaan geen creditcardgegevens op en hebben geen toegang tot uw creditcardinformatie.
Navraag
Misschien vind je het ook leuk
FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
Voor bepaalde toepassingen zijn mogelijk aanvullende intellectuele eigendomsrechten van derden vereist.