Description
Hieff™ Taq DNA polymerase is a thermostable recombinant wild type DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning.
Features
- 5'→3' DNA polymerase activity
- 5'→3' exonuclease activity
- Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less
Applications
Genotyping, colony PCR and other conventional PCR
Specifications
| Polymerase | Taq DNA polymerase |
| Molecular Weight | 94 KDa |
| Unit Definition | Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. |
Components
| Components No. | Name | 10101ES80 (1, 000 U) | 10101ES92 (10, 000 U) |
| 10101-A | 10×Taq Buffer (Mg2+ Free) | 4×1 mL | 4×10 mL |
| 10101-B | 25 mM MgCl2 | 2×1 mL | 2×10 mL |
| 10101-C | Hieff™ Taq DNA Polymerase (5 U/μL) | 200 μL | 2×1 mL |
Storage
The product should be stored at -25℃ ~ 15℃ for two years.
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