Description
dUTP, or 2 '-deoxyuridine 5'-triphosphate, is commonly used in PCR, qPCR, RT-PCR and RT-qPCR to replace dTTP, so that the product of amplification contains uracil. When the UDG is added to the amplification reaction system, the uracil of any contaminated amplification products can be removed, so as to avoid the false positive.
This product is in the form of solution sodium salt, prepared with ultrapure water, and adjusted to pH value of 7.0 with high-purity NaOH solution, with a concentration of 100 mmol/L.
The purity of dUTP was ≥99% (HPLC), and there was no DNase and RNase pollution. This product is ready to use and can be directly used in PCR and other conventional molecular biological reactions.
Features
- Greater than 99% purity—confirmed by HPLC
- Free of human and E. coli DNA
- Highly stable
Applications
- Long range PCR (40 kb)
- cDNA synthesis and RT-PCR
- Real-time PCR
- Standard PCR
- High fidelity PCR
Specifications
| Concentration | 100 mM |
| CAS NO. | 102814-08-4 |
| Form | Liquid |
| Purity | >99% |
| Label or Dye | Unlabeled |
| For Use With (Application) | PCR Amplification |
Components
| Components No. | Name | 10128ES74 | 10128ES80 | 10128ES96 | 10128ES97 |
| 10128 | dUTP, 100 mmol/L Solution | 400 μL | 1 mL | 25 mL | 400 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.