Description
UTP, Uridine-5'-triphosphate, can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, UTP is involved in galactose metabolism. The activated form of UDP-galactose is converted to UDP-glucose, which participates in the glycogen synthesis pathway. This product is a transparent colorless aqueous solution prepared from UTP trisodium salt, and free from DNase and RNase contamination. This product is produced in accordance with GMP process requirements and provided in liquid form.
Feature
- Validated, product-specific process and analytical methods
- Product-specific stability
- Documentation follows applicable GMP guidelines
- AOF production process and raw materials (TSE & BSE)
- Nitrosamine statement
- Regulatory support documents available
- Large-scale production
- Nucleotides in the multiple salt form（Na+, Tris, etc) always available to meet different downstream application needs
Application
- RNA synthesis and amplification
- Building block for in vitro transcription
Specification
| CAS No | 63-39-8 (free acid); 19817-92-6 (3Na salt) |
| Formula | C9H12N2Na3O15P3 |
| Molecular Weight | 550.09 g/moL |
| Purity (HPLC) | ≥ 99% |
| Content | 100 mM ± 3 mM |
| Structure |  |
Component
| Components No. | Name | 10131ES03 | 10131ES10 | 10131ES60 |
| 10131 | UTP Solution GMP-grade (100 mM) | 1 mL | 10 mL | 100 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.
Figures
- Standard RNA Synthesis
Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.
- Capped RNA Synthesis
Figure 2. Synthesis of capped RNA in vitro.
The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.