Description
Description
Agarose is a purified linear galactoglycan hydrophilic colloid derived from agar or agar-containing algae. It is a linear polymer composed of β-d-galactosyl (1-4) linked to 3, 6-dehydrated-L-galactosyl. As a gel reagent, it is commonly used for routine nucleic acid analysis by gel electrophoresis or blotting (such as Northern or Southern) and for protein applications, such as radiative immunodiffusion (RID) assay.
This product is PCR-grade high sieving agarose, which offers high resolution for PCR products and small DNA fragments. It is suitable for separating DNA fragments ranging from 20 bp to 800 bp, with separation effects comparable to those of polyacrylamide. In addition, it has the following features:
1) Easy to dissolve, resulting in clearer gel solutions, and gels up to 5% can be prepared;
2) Very low background;
3) Stable quality.
Features
Low EEO
Applications
Gel electrophoresis
Separation of 20 - 800 bp DNA fragments
Components
|
Name |
10221ES08 |
10221ES25 |
10221ES60 |
|
High Sieving Agarose (PCR Grade) |
5 g |
25 g |
100 g |
Specifications
|
39346-81-1 |
|
|
Appearance |
White to off-white powder |
|
Gel Strength (1.5%) |
≥750 (g/cm2) |
|
Gel Point (1.5%) |
≤33℃ |
|
Melting Point (1.5%) |
≤70℃ |
|
EEO (-mr) |
≤0.10 |
|
Sulfate (%) |
≤0.10% |
|
Moisture |
≤10% |
|
Ash Content |
≤0.5% |
|
DNase |
None Detected |
|
RNase |
None Detected |
|
Protease |
None Detected |
|
Endonuclease |
None Detected |
Storage
The Agarose products should be stored at room temperature for 5 years.
Agarose Concentration and DNA Separation Range
|
Size of Linear DNA Fragments (bp) |
20-250 bp |
50-500 bp |
100-1200 bp |
500-2000 bp |
|
Agarose Concentration (%) |
5.0 |
4.0 |
3.0 |
2.0 |
Related Citations & References:
[1] Li Z, Wang M, Fang H, et al. Solid-liquid interface adsorption of antibiotic resistance plasmids induced by nanoplastics aggravates gene pollution in aquatic ecosystems. Environ Pollut. 2023. doi:10.1016/j.envpol.2022.120456. (IF=10.366)
Documents:
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