Recombinant Deoxyribonuclease I (DNase I, RNase-free) (2 U/μL) -10325ES

DNase I is an endonuclease that can digest single- or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono- and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group.The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca²⁺ and can be activated by divalent metal ions such as Co²⁺, Mn²⁺, Zn²⁺, etc. In the presence of Mg²⁺, DNase I can randomly cleave any site of double-stranded DNA; while in the presence of Mn²⁺, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding. It can be used for the processing of various RNA samples.

Specifications

Cat.No.	10325ES80 / 10325ES90
Size	1,000 U /5,000 U
Expression Host	Recombinant E. coli with Dnase I gene
Unit Definition	The amount of enzyme required to completely degrade 1 μg of plasmid DNA at 37℃ for 10 mins

Components

Components No.	Name	10325ES80	10325ES90
10325-A	Recombinant DNase I (RNase-free) -2 U/μL	500 μL	5×500 μL
10325-B	DNase I Reaction Buffer (10×)	1 mL	5×1 mL

Storage

This product should be stored at -25~-15℃ for 2 years. Please avoid repeated freeze-thaw.

Notes

1. DNase I is sensitive to physical denaturation; when mixing, gently reverse the test tube and shake well, do not shake vigorously.
2. The enzyme should be stored in an ice box or on an ice bath when used, and should be stored at -20℃ immediately after use.
3. This product is for research use only.
4. 4. Please operate with lab coats and disposable gloves，for your safety.

Documents:

Safety Data Sheet

10325_MSDS_HB220421_EN_1652931562479.PDF

10325_MSDS_HB220421_EN_1652931562479.PDF

Manuals

10325-Recombinant DNase I (RNase-free)-Ver.EN20230606.pdf

Citations & References:

[1] Qin H, Gui Y, Ma R, et al. miR-1258 Attenuates Tumorigenesis Through Targeting E2F1 to Inhibit PCNA and MMP2 Transcription in Glioblastoma. Front Oncol. 2021;11:671144. Published 2021 May 17. doi:10.3389/fonc.2021.671144(IF:6.244)

[2] Mei X, Gao M, Huang T, et al. Comparative analysis of testis transcriptome between a genetic male sterile line (GMS) and its wild-type 898WB in silkworm, Bombyx mori. Comp Biochem Physiol Part D Genomics Proteomics. 2022;42:100961. doi:10.1016/j.cbd.2022.100961(IF:2.674)