Description
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids with a relative molecular weight of 29.3 kDa.The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide .Proteinase K is widely used for the preparation of chromosomal DNA , and removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines.Denaturants such as SDS (1%) can increase their activity.It is commonly used at working concentrations of 50-100 μg/mL.
Features
Enzyme activity ≥30 U/mg
No RNase residue
No DNase residue
Applications
Nucleic Acid Extraction & Purification
Remove endogenous nucleases during the preparation of DNA and RNA
Prepare tissue sections for in - situ hybridization.
Specifications
|
Cat.No. |
10412ES03 / 10412ES10 / 10412ES25 /10412ES76 |
|
Size |
1 mL /10 mL/ 25 mL / 500 mL |
Shipping and Storage
This product should be stored at 2~8℃ for 2 years.For longer storage, it can be stored at -25~-15℃.
Product Properties
|
Source |
Yeast |
|
CAS NO. |
39450-01-6 |
|
Molecular Weight |
29 kD |
|
Units/mg |
≥30 U/mg |
|
Appearance |
Liquid |
|
Purity |
≥95% SDS-PAGE |
|
DNase Contamination |
NO |
|
RNase Contamination |
NO |
|
Unit Definition |
At 37°C, pH 7.5, every minute released 1 μM tyrosine required Proteinase K is defined as 1 U |
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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