Description
DNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones). The optimum pH range is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions, such as Co2+, Mn2+, Zn2+, etc. 5 mM Ca2+ can protect the enzyme from being hydrolyzed. In the presence of Mg2+, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn2+, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.
This product is produced in accordance with GMP process requirements, and the product is provided in liquid form.
Feature
- DNA specific Endonuclease
- Degrades double-stranded and single-stranded DNA
- Products are short oligos with 5'-phosphate and 3'-OH
Application
- Removal of contaminating genomic DNA from RNA samples
- Degradation of DNA templates in transcription reactions
Specification
| Source | Recombinant E. coli with DNase I gene |
| Optimum temperature | 37℃ |
| Storage Buffer | 10 mM Tris-HCl pH 7.6，2 mM CaCl2, 50% (v/v) Glycerol |
| Unit Definition | The amount of enzyme required to completely degrade 1 μg of plasmid DNA within 10 min at 37°C. (The reaction buffer is: 10 mM Tris-HCl pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 μg plasmid DNA) |
Components
| Components No. | Name | 10611ES76 (500 U) | 10611ES84 (2,000 U) | 10611ES92 (10,000 U) |
| 10611 | Deoxyribonuclease I (DNase I) GMP-grade (2 U/μL) | 250 μL | 1 mL | 5 mL |
Shipping and Storage
Deoxyribonuclease I (DNase I) GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figure 1. DNase I from YEASEN could effectively remove DNA template during transcription，compared with competitor brands.
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