Description
mRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.
This product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo/in vitro.
Feature
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, Rnases
Application
- 2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo
- mRNA cap-1 structure may improve expression
Specification
| Source | Recombinant E.coli with Cap 2´-O-Methyltransferase gene |
| Optimum Temperature | 37℃ |
| Storage Buffer | 20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v/v) glycerin, 0.1% (v/v) Trion X-100 |
| Unit Definition | One unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C. |
Components
| Components No. | Name | 10612ES92 (10,000 U) | 10612ES97 (50,000 U) | 10612ES98 (250,000 U) | 10612ES99 (20 MU) |
| 10612 | mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U/μl) | 200 μL | 1 mL | 5 mL | 400 mL |
Shipping and Storage
The mRNA Cap 2´-O-Methyltransferase GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figures 1. The relationship between capping efficiency and 2’-O-Methyltransferase quantity