Description
RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.
Feature
- Improved resistance to oxidation, compared to human/porcine RNase inhibitor
- Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
- Isolated from a recombinant source
- Tested for the absence of DNases and RNases
Application
- RT-PCR
- cDNA synthesis
- In vitro transcription/translation
- Enzymatic RNA labeling reaction
- Other applications where the integrity of RNA is important
Specification
| Source | Recombinant E.coli with Murine RNase inhibitor gene |
| Storage Buffer | 20 mM Hepes KOH，150 mM KCl，8 mM DTT，0.5%（v/v）NP 40，0.5%（v/v）Tween20，50%（v/v）Glycerol，pH 7.5 ±0.2 (25℃) |
| Unit Definition | The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit |
Components
| Components No. | Name | 10621ES10 (10 KU) | 10621ES20 (20 KU) | 10621ES60 (100 KU) | 10621ES99 (1 MU) |
| 10621 | Murine RNase inhibitor GMP-grade (40 U/μL) | 250 μL | 500 μL | 2.5 mL | 25 mL |
Shipping and Storage
The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
[1] Shen W, Wang R, Fan Q, Li Y, Cheng Y. Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038/s41434-020-0151-y(IF:4.128)