Description
This product is a type IIS restriction endonuclease derived from the recombinant protein encoded by the BsaI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GGTCTCN1/N5-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.
This product is produced in accordance with GMP process requirements and provided in a liquid form.
Features
1. Complete FDA DMF certification (MF038306)
2. GMP-grade, no animal-derived ingredients, strict quality control and production in strict accordance with GMP standards to ensure the introduction of no animal-derived ingredients.
3. High enzyme cutting efficiency, low star activity, and good lethality. It has high enzyme cutting efficiency and no star activity after 16 hours of reaction at 37°C. It has good lethality after enzyme digestion-ligation-re-digestion test.
4. Superior performance application performance is comparable to competing products
Applications
Plasmid linearization for mRNA vaccine production,
Lentivirus/adenovirus packaging vector construction,
Enzyme digestion verification of plasmid preparation,
Golden Gate assembly
DNA labeling
Specifications
|
Expression Host |
Recombinant E. coli with Bas I gene |
|
Reaction Temperature |
37℃ |
|
Storage Buffer |
10mM Tris-HCl, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.2mg/ml OsrHSA pH 7.4±0.2 (25℃) |
|
Unit Definition |
1 unit: The amount of enzyme required to digest 1 μg of substrate DNA within 1 h at 37℃ in a 50 μL system. |
|
Application |
1.Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G); 2.Digestion of DNA to obtain specific sticky ends； 3.Linearize plasmid template before in-vitro transcription. |
Components
|
Components No.
|
Name |
10661ES03 (1 KU) |
10661ES10 (10 KU) |
10661ES60 (100 KU) |
|
10661 |
Bsa I GMP-grade (20 U/μL) |
50 μL |
500 μL |
5 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for two years.
Figures
Figure1. No non-specific nuclease residues test
20 U BsaI was incubated with substrate DNA at 37°C for 1 h and 16 h, and DNA band changes were compared by agarose gel electrophoresis. The results showed that there was no non-specific nuclease residue in BsaI.
Figure2. The end integrity is good and the effect is consistent with imported brands
When BsaI and substrate DNA were incubated in the enzyme digestion reaction system for 16 hours at 37°C, no non-specific degradation of the substrate caused by star activity was detected, indicating that there was no star activity after 16 hours of incubation with BsaI.
Documents:
Safety Data Sheet
Manuals
10661_Manual_HB20241220_EN.PDF
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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