Description
This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form.
Feature
- Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
- Restriction Enzyme Cut Site: GCTCTTC (1/4)
- Digestion of DNA to obtain specific sticky ends
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, RNases
Application
- Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
- Linearize plasmid template before in vitro transcription
- Restriction digest
Specification
| Expression Host | Recombinant E. coli with BspQI gene |
| Reaction Temperature | 50℃ |
| Storage Buffer | 20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% Glycerol |
| Unit Definition | 1 unit: The amount of enzyme required to digest 1 μg of λDNA within 1 h at 50℃ in a 50 μL system |
Components
| Components No. | Name | 10664ES76 (500 U) | 10664ES86 (2,500 U) | 10664ES92 (10 KU) | 10664ES98 (100 KU) |
| 10664 | BspQI GMP-grade (10 U/μL) | 50 μL | 250 μL | 1 mL | 10 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figure1. The performance of YEASEN BspQI is superior.
In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M: DNA marker
C: The control group without BspQI treatment
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.