Description
This product is an optimized 10×Digestion buffer for different restriction enzymes to ensure optimal (100%) digestion activity. The formulation of the product: 500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl2, 1 mg/mL OsrHSA, pH 7.9 at 25°C. This product is produced in accordance with GMP process requirements and provided in a liquid form.
Application
- Buffer for different restriction enzymes to ensure optimal (100%) digestion activity
Specification
| Format | Liquid |
| Concentration | 10X |
| Formulation | 500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl2, 1 mg/mL OsrHSA, pH 7.9 at 25°C |
Components
| Components No. | Name | 10667ES03 | 10667ES10 | 10667ES50 |
| 10667 | 10×Digestion buffer 3 GMP-grade | 1 mL | 10 mL | 50 mL |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
Figures
Figure1. The performance of YEASEN BspQI is superior.
In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.
M: DNA marker
C: The control group without BspQI treatment