Description
UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature.
Applications
1. Remove aerosol contamination from PCR products containing dU.
2. Remove single-stranded or double-stranded DNA uracil bases.
Specifications
|
Unit Definition |
The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U). |
|
Deactivation method |
94℃, 2-5 minutes. |
Components
|
No. |
Name |
10707ES60 (100 U) |
10707ES76 (500 U) |
10707ES90 (5 KU) |
10707ES92 (10 KU) |
|
10707 |
Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL) |
100 μL |
500 μL |
5 mL |
10 mL |
Shipping and Storage
Ice pack transport. Store at 4°C, valid for 6 months.Precautions
1. For your safety and health, please wear a lab coat and disposable gloves.
2. Thermosensitive UDG is active in most PCR reaction buffers.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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